Embryonic expression of pericentrin suggests universal roles in ciliogenesis
Embryonic expression of pericentrin suggests universal roles in ciliogenesis
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DOI：
10.1007/s00427-006-0065-8
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发表时间：
2006-09-01
影响因子：
2.4
通讯作者：
Ogawa, Norio
中科院分区：
文献类型：
--
作者：
Miyoshi, Ko;Onishi, Kazunari;Ogawa, Norio
Pericentrin（Pcnt）是一种巨大的卷曲螺旋蛋白，已知其介导微管组织化。最近有报道，有丝分裂特异性中心体锚定的γ微管蛋白复合物的Pcnt的行为，以控制有丝分裂纺锤体组织，虽然鲜为人知的是在体内表达的Pcnt。在这项研究中，我们研究了Pcnt在小鼠胚胎中的表达。原位杂交分析显示Pcnt在整个胚胎的静止期G（0）细胞中优先表达，而在增殖期细胞中的表达水平出乎意料地低，这表明Pcnt可能在有丝分裂增殖中不起重要作用。免疫荧光分析证实Pcnt蛋白在G（0）期细胞中优先表达。此外，Pcnt被定位于多个胚胎组织中的初级纤毛的基部，与最近的一项研究一致，该研究表明Pcnt参与使用培养的哺乳动物细胞的初级纤毛形成。
Pericentrin (Pcnt) is a giant coiled-coil protein known to mediate microtubule organization. It has been recently reported that mitosis-specific centrosomal anchoring of gamma tubulin complexes by Pcnt acts to control mitotic spindle organization, though little is known about the in vivo expression of Pcnt. In this study, we investigated Pcnt expression in mouse embryos. In situ hybridization analysis revealed preferential expression of Pcnt in quiescent G(0) phase cells throughout the embryo with an unexpectedly low expression level in proliferating cells, suggesting that Pcnt might not play an important role in mitotic proliferation. Immunofluorescence analysis confirmed preferential expression of the Pcnt protein in G(0) phase cells. Moreover, Pcnt was shown to be localized to the base of primary cilia in multiple embryonic tissues, in agreement with a recent study demonstrating the involvement of Pcnt in primary cilia formation using cultured mammalian cells.