Monocyte-macrophage membrane possesses free radicals scavenging activity: stimulation by polyphenols or by paraoxonase 1 (PON1)
Monocyte-macrophage membrane possesses free radicals scavenging activity: stimulation by polyphenols or by paraoxonase 1 (PON1)
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DOI：
10.3109/10715762.2013.765562
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发表时间：
2013-04-01
影响因子：
3.3
通讯作者：
Aviram, M.
中科院分区：
文献类型：
--
作者：
Rosenblat, M.;Elias, A.;Aviram, M.
在目前的研究中，我们分析了自由基清除活性的单核细胞-巨噬细胞的存在或不存在的抗氧化剂，如多酚或对氧磷酶1（PON 1）。THP-1人单核细胞系、鼠J774A.1巨噬细胞以及人原代单核细胞具有清除自由基的能力，如通过1-二苯基-2-苦基-肼（DPPH）测定所测量的。这种效应（可能归因于细胞膜）依赖于细胞数量和孵育时间。J774A.1巨噬细胞与乙酰化低密度脂蛋白（Ac-LDL）、极低密度脂蛋白（VLDL）或自由基发生剂AAPH孵育后，细胞的脂质过氧化物含量和对氧磷酶2（PON 2）活性显著增加。虽然未处理的细胞降低DPPH吸光度65%，但Ac-LDL-，VLDL-或AAPH-处理的细胞分别仅降低33%，30%或45%。接下来，我们分析了用抗氧化剂如多酚或P0 N 1富集J774A.1巨噬细胞对细胞的自由基清除活性的影响。未处理的细胞减少DPPH吸光度的50%，而维生素E，安石榴苷或PJ处理的细胞显着进一步降低它，由75%。类似地，在PON 1处理的细胞中，DPPH吸光度进一步降低63%，与PON 1催化活性增加23%相关。在氧化应激下的细胞中[用AAPH-或氧化LDL处理]，PON 1活性降低了31%或40%，与未处理细胞孵育的PON 1中观察到的活性相比。我们的结论是，单核细胞-巨噬细胞具有自由基清除活性，这是降低动脉粥样硬化条件下，并增加细胞富集与有效的抗氧化剂，如营养多酚，或PON 1。
In the current study, we analysed free radicals scavenging activity of monocytes-macrophages in the absence or presence of antioxidants such as polyphenols or paraoxonase 1 (PON1). THP-1 human monocytic cell line, murine J774A.1 macrophages, as well as human primary monocytes have the capability to scavenge free radicals, as measured by the 1-diphenyl-2-picryl-hydrazyl (DPPH) assay. This effect (which could be attributed to the cell's membrane) was cell number and incubation time dependent. Upon incubation of J774A.1 macrophages with acetylated LDL (Ac-LDL), with VLDL, or with the radical generator, AAPH, the cells' lipid peroxides content, and paraoxonase 2 (PON2) activity were significantly increased. While non-treated cells decreased DPPH absorbance by 65%, the Ac-LDL-, VLDL- or AAPH-treated cells, decreased it by only 33%, 30%, or 45%, respectively. We next analysed the effect of J774A.1 macrophage enrichment with antioxidants, such as polyphenols or PON1 on the cells' free radicals scavenging activity. Non-treated cells decreased DPPH absorbance by 50%, whereas vitamin E-, punicalagin- or PJ-treated cells significantly further decreased it, by 75%. Similarly, in PON1-treated cells DPPH absorbance was further decreased by 63%, in association with 23% increment in PON1 catalytic activity. In cells under oxidative stress [treated with AAPH-, or with oxidized LDL], PON1 activity was decreased by 31% or 40%, as compared to the activity observed in PON1 incubated with non-treated cells. We conclude that monocytes-macrophages possess free radicals scavenging activity, which is decreased under atherogenic conditions, and increased upon cell enrichment with potent antioxidants such as nutritional polyphenols, or PON1.