An extra-domiciliary method of delivering entomopathogenic fungus, Metharizium anisopliae IP 46 for controlling adult populations of the malaria vector, Anopheles arabiensis
An extra-domiciliary method of delivering entomopathogenic fungus, Metharizium anisopliae IP 46 for controlling adult populations of the malaria vector, Anopheles arabiensis
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DOI：
10.1186/1756-3305-3-18
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发表时间：
2010-03-16
影响因子：
3.2
通讯作者：
Okumu, Fredros O.
中科院分区：
文献类型：
--
作者：
Lwetoijera, Dickson W.;Sumaye, Robert D.;Okumu, Fredros O.
真菌生物杀虫剂有可能显著降低疟疾病媒的密度以及相关的疟疾传播。在以前的田间试验中，昆虫病原真菌是从人类住宅内递送的，其中其功效受到目标蚊子的低感染率、在房屋内喷洒真菌的高成本以及与在房屋内引入真菌分生孢子相关的潜在公共卫生问题的限制。在这里，我们已经证明，绿僵菌IP 46，在一个室外气味诱饵站（OBS）内传递，可以感染和缓慢杀死高比例的野生成年疟疾媒介，阿拉伯按蚊进入和离开OBS。这项研究，在坦桑尼亚农村进行，表明通过使用3.9 × 10（10）分生孢子/m2的浓度，超过95%飞进和飞出OBS的蚊子在接触后14天内死亡。至少有86%的蚊子尸体的感染记录与暴露的An的日常生存的概率显着降低。结果表明，两种处理的分生孢子数量分别为：低分生孢子数量（檐板+1个棉板; HR = 2.65，P < 0.0001）和高分生孢子数量（檐板+2个棉板; HR = 2.32，P < 0.0001）。我们的结论是，昆虫病原真菌对野生疟疾病媒的高感染率和疟疾传播的可能显着中断，可以实现，如果真菌提供使用最佳位置的室外气味诱饵站。
Fungal biopesticides have the potential to significantly reduce densities of malaria vectors as well as associated malaria transmission. In previous field trials, entomopathogenic fungus was delivered from within human dwellings, where its efficacy was limited by low infection rates of target mosquitoes, high costs of spraying fungus inside houses, and potential public health concerns associated with introducing fungal conidia inside houses. Here we have demonstrated that Metarhizium anisopliae IP 46, delivered within an extra-domiciliary odor-baited station (OBS), can infect and slowly-kill a high proportion of the wild adult malaria vector, Anopheles arabiensis which entered and exited the OBS. This study, carried out in rural Tanzania, showed that by using a concentration of 3.9 x 10(10) conidia/m(2), more than 95% of mosquitoes that flew in and out of the OBS died within 14 days post-exposure. At least 86% infection of mosquito cadavers was recorded with a significant reduction in the probability of daily survival of exposed An. arabiensis in both treatments tested: low quantity of conidia (eave baffles plus one cotton panel; HR = 2.65, P < 0.0001) and high quantity of conidia (eave baffles plus two cotton panels; HR = 2.32, P < 0.0001). We conclude that high infection rates of entomopathogenic fungi on wild malaria vectors and possibly significant disruption of malaria transmission can be achieved if the fungus is delivered using optimally located outdoor odor-baited stations.