Red blood cell storage in SAGM and AS3: a comparison through the membrane two-dimensional electrophoresis proteome
Red blood cell storage in SAGM and AS3: a comparison through the membrane two-dimensional electrophoresis proteome
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DOI：
10.2450/2012.008s
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发表时间：
2012-05-01
影响因子：
3.7
通讯作者：
Zolla, Lello
中科院分区：
文献类型：
--
作者：
D'Amici, Gian Maria;Mirasole, Cristiana;Zolla, Lello
背景。 SAGM是目前欧洲使用的标准添加剂溶液，而AS-3是第三种在美国获得许可的添加剂溶液，也是加拿大部分地区使用的添加剂溶液。尽管 AS-3 基于盐水-腺嘌呤-葡萄糖溶液，但它还含有柠檬酸盐和磷酸盐。众所周知，CPD-SAGM 中红细胞浓缩物的储存会导致一系列储存损伤的积累，包括膜蛋白断裂和囊泡形成，正如我们之前可以通过二维凝胶电泳确定的那样。材料和方法。通过 2D-SDS-IEF-聚丙烯酰胺凝胶电泳，我们对储存在 CPD-SAGM 或 CP2D-AS-3 中的白细胞过滤浓缩物的红细胞膜进行了时间过程分析（储存第 0、21 和 42 天）。结果和讨论。从本研究中可以看出，在 AS-3 存在的情况下储存的红细胞的膜蛋白谱似乎与之前关于 SAGM 储存的对应物的报道略有不同（更好）。然而，由于第 21 天碎片的存在而导致总膜点数量增加，而第 42 天则显着减少，这表明存在一种普遍现象，而本研究中研究的两种添加剂溶液均未能有效解决该现象。结论。为了进一步深入研究 AS-3 中储存的红细胞的储存损伤问题，未来分析储存过程中的代谢变化也会很有趣。
Background. SAGM is currently the standard additive solution used in Europe, while AS-3 is the third additive solution that has been licensed in the USA, and is also the one used in part of Canada. Although AS-3 is based on a saline-adenine-glucose solution, it also contains citrate and phosphate. Storage of red blood cell concentrates in CPD-SAGM is known to lead to the accumulation of a wide series of storage lesions, including membrane protein fragmentation and vesiculation, as we could previously determine through 2-dimensional gel electrophoresis.Materials and methods. Through 2D-SDS-IEF-polyacrilamide gel electrophoresis we performed a time course analysis (day 0, 21 and 42 of storage) of red blood cell membranes from leukocyte-filtered concentrates either stored in CPD-SAGM or CP2D-AS-3.Results and discussion. From the present study it emerges that the membrane protein profile of red blood cells stored in presence of AS-3 appears to be slightly different from (better than) previous reports on SAGM-stored counterparts. However, the increase of total membrane spot number due to the presence of fragments at day 21 and the significant decrease at day 42 are suggestive of a universal phenomenon which is not efficiently tackled by either of the two additive solutions investigated in the present study.Conclusion. To further delve into the storage lesion issue for RBCs stored in AS-3, it would be interesting in the future to assay metabolic changes over storage progression as well.