Rapid selection of transgenic C. elegans using antibiotic resistance
Rapid selection of transgenic C. elegans using antibiotic resistance
复制标题
DOI：
10.1038/nmeth.1495
复制
发表时间：
2010-09-01
期刊：
影响因子：
48
通讯作者：
Lehner, Ben
中科院分区：
文献类型：
--
作者：
Semple, Jennifer I.;Garcia-Verdugo, Rosa;Lehner, Ben
秀丽隐杆线虫是生物学中一种重要的模式生物，但迄今为止还没有成功地证明该物种的抗生素选择标记。我们已经开发了一种使用嘌呤霉素的选择系统，可以快速和容易地分离出大量的转基因蠕虫。这种方法在选择单拷贝转基因时足够强大，不需要任何特定的遗传背景，并且在C. briggsae中也有效。
Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.