Coordinate repression of regulators of embryonic identity by PICKLE during germination in Arabidopsis
Coordinate repression of regulators of embryonic identity by PICKLE during germination in Arabidopsis
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DOI：
10.1046/j.1365-313x.2003.01783.x
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发表时间：
2003-07-01
期刊：
影响因子：
7.2
通讯作者：
Ogas, J
中科院分区：
文献类型：
--
作者：
Rider, SD;Henderson, JT;Ogas, J
在被子植物中，萌发代表了一个重要的发育转变，在此期间，胚胎身份被抑制，营养身份出现。PICKLE（PKL）编码一种抑制叶子叶1（LEC 1）表达所必需的CHD 3-染色质重塑因子，LEC 1是胚胎发生的中心调节因子。候选基因的方法和微阵列分析鉴定了9个额外的基因，表现出PKL依赖性抑制发芽过程中的表达。所有三种叶子叶基因（LEC 1、LEC 2和FUS 3）的转录物都表现出PKL依赖性抑制，并且所有三种转录物在不适当表达胚性状的PK 1主根（泡菜根）中升高超过100倍.表现出PKL依赖性调节的三个其它基因具有与合子或体细胞胚胎发生相关的表达模式，并且一个基因编码优先在长角果中表达的推定的Lin-11、Isl-1、MEC-3（LIM）结构域转录调节因子.在萌发过程中表现出PKL依赖性抑制的基因在发育的其他阶段不一定受PKL的调控。我们的数据表明，PKL选择性地调节一套基因在发芽过程中抑制胚胎的身份。特别是，我们建议PKL作为一个主调节器的叶子叶基因，这些基因的联合去阻遏很可能有助于显着表达的胚胎身份PKL幼苗。
In angiosperms, germination represents an important developmental transition during which embryonic identity is repressed and vegetative identity emerges. PICKLE (PKL ) encodes a CHD3-chromatin-remodeling factor necessary for the repression of expression of LEAFY COTYLEDON1 (LEC1 ), a central regulator of embryogenesis. A candidate gene approach and microarray analysis identified nine additional genes that exhibit PKL -dependent repression of expression during germination. Transcripts for all three LEAFY COTYLEDON genes, LEC1 , LEC2 , and FUS3 , exhibit PKL -dependent repression, and all three transcripts are elevated more than 100-fold in pkl primary roots that inappropriately express embryonic traits (pickle roots). Three other genes that exhibit PKL -dependent regulation have expression patterns correlated with zygotic or somatic embryogenesis, and one gene encodes a putative Lin-11, Isl-1, MEC-3 (LIM) domain transcriptional regulator that is preferentially expressed in siliques. Genes that exhibit PKL -dependent repression during germination are not necessarily regulated by PKL at other points in development. Our data suggest that PKL selectively regulates a suite of genes during germination to repress embryonic identity. In particular, we propose that PKL acts as a master regulator of the LEAFY COTYLEDON genes, and that joint derepression of these genes is likely to contribute substantially to expression of embryonic identity in pkl seedlings.