The Myxococcus xanthus wbgB gene encodes a glycosyltransferase homologue required for lipopolysaccharide O-antigen biosynthesis.
The Myxococcus xanthus wbgB gene encodes a glycosyltransferase homologue required for lipopolysaccharide O-antigen biosynthesis.
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黄色粘球菌 wbgB 基因编码脂多糖 O 抗原生物合成所需的糖基转移酶同源物。
DOI：
10.1007/s002030000226
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发表时间：
2000
影响因子：
2.8
通讯作者：
Shi,W
中科院分区：
文献类型：
--
作者：
Yang,Z;Guo,D;Bowden,MG;Sun,H;Tong,L;Li,Z;Brown,AE;Kaplan,HB;Shi,W
Myxococcus xanthusis a gram-negative soil bacterium that initiates a complex developmental program in response to starvation. A transposon insertion (Tn5-lacΩ109) mutant with developmental deficiencies was isolated and characterized in this study. A strain containing this insertion mutation in an otherwise wild-type background showed delayed developmental aggregation for about 12 h and sporulated at 1–2% of the wild-type level. Tn5-lacΩ109 was found to have disrupted theM. xanthus wbgBgene, which is located 2.1 kb downstream of theM. xanthuslipopolysacharide (LPS) O-antigen biosynthesis geneswzm wzt wbgA. The deduced polypeptide sequence of WbgB shares significant similarity with bacterial glycosyltransferases includingM. xanthusWbgA. ThewbgB::Tn5-lacΩ109 mutant was found to be defective in LPS O-antigen synthesis by immunochemical analysis. Further mutational analysis indicated that the defects of thewbgB::Tn5-lacΩ109 mutant were not the result of polar effects on downstream genes. Various motility assays demonstrated that the Tn5-lacΩ109 mutation affected both social (S) and adventurous (A) gliding motility ofM. xanthuscells. The pleiotrophic effects ofwbgBmutations indicate the importance of LPS O-antigen biosynthesis for various cellular functions inM. xanthus.
