Bacterial Community Profiling of Cryogenically Ground Samples from the Apical and Coronal Root Segments of Teeth with Apical Periodontitis
Bacterial Community Profiling of Cryogenically Ground Samples from the Apical and Coronal Root Segments of Teeth with Apical Periodontitis
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DOI：
10.1016/j.joen.2008.12.022
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发表时间：
2009-04-01
影响因子：
4.2
通讯作者：
Rosado, Alexandre S.
中科院分区：
文献类型：
--
作者：
Alves, Flavio R. F.;Siqueira, Jose F., Jr.;Rosado, Alexandre S.
位于感染根管的根尖部分的细菌被认为直接参与了根尖周炎的发病机制。本研究旨在描述和进一步比较感染根管的根尖段和中冠段的细菌群落。对附着性根尖周炎病变的拔除牙切取两个根段，分别代表根尖1/3和冠部2/3。对根碎片进行低温研磨处理。从根粉样品中提取DNA，作为模板，使用基于16S核糖体RNA基因的半套式聚合酶链式反应/变性梯度凝胶电泳法进行细菌群落分析。原发感染牙根尖标本的平均条带数为28条，范围为18-48条，而中部/冠状样本的条带数也为28条，范围为19-36条。结果表明，感染根管的根尖1/3和中部/冠状1/3的细菌群落分布是不同的。个体间(来自相同区域但来自不同患者的样本)和个体内(来自同一牙齿不同区域的样本)的比较都观察到了高度的变异性。用于制备和分析样本的方法非常有效地揭示了以前没有预料到的牙髓细菌群落的广泛多样性，特别是在受感染的根管的根尖部分。(J Endod 2009；35：486-492)
Bacteria located at the apical part of infected root canals are arguably directly involved in the pathogenesis of apical periodontitis. This study was conducted to profile and further compare the bacterial communities established at the apical and middle/coronal segments of infected root canals. Extracted teeth with attached apical periodontitis lesions were sectioned so as to obtain two root fragments representing the apical third and the coronal two thirds. Root fragments were subjected to a cryogenic grinding approach. DNA was extracted from root powder samples and used as a template for bacterial community profiling using a 16S ribosomal RNA gene-based seminested polymerase chain reaction/denaturing gradient gel electrophoresis approach. The mean number of bands in apical samples from teeth with primary infections was 28, ranging from 18 to 48, whereas in the middle/coronal samples, it was also 28, ranging from 19 to 36. Findings showed that the profile of bacterial community colonizing the apical third of infected root canals is as diverse as that occurring at the middle/coronal thirds. A high variability was observed for both interindividual (samples from the same region but from different patients) and intraindividual (samples from different regions of the same tooth) comparisons. The methodology used to prepare and analyze samples was highly effective in disclosing a previously unanticipated broad diversity of endodontic bacterial communities, especially at the apical part of infected root canals. (J Endod 2009;35:486-492)