Autoantigens in ovarian autoimmunity associated with unexplained infertility and premature ovarian failure.
Autoantigens in ovarian autoimmunity associated with unexplained infertility and premature ovarian failure.
复制标题
DOI：
10.1016/j.fertnstert.2010.04.012
复制
发表时间：
2010-12
影响因子：
6.7
通讯作者：
Luborsky JL
中科院分区：
文献类型：
--
作者：
Edassery SL;Shatavi SV;Kunkel JP;Hauer C;Brucker C;Penumatsa K;Yu Y;Dias JA;Luborsky JL
鉴定与卵巢自身抗体相关的卵巢自身抗原。假设产生前瞻性研究。城市不孕不育转诊中心和学术研究机构。不孕症患者74例，卵巢早衰患者19例，健康对照组16例。没有。自身抗原的鉴定。为确定卵巢自身免疫的主要抗原，采用免疫分析和一维免疫印迹法对74例不明原因不孕症患者血清进行卵巢自身抗体（AOA）筛查。大多数血清在50- 56 kDa处有免疫反应。用6份有代表性的不孕症阳性血清，通过双向免疫印迹和质谱鉴定40- 60 kD之间的抗原。抗原包括醛（视网膜）脱氢酶（ALDH 1A 1、ALDH 1A 2、ALDH 7A 1）、蛋白二硫键异构酶A3（PDIA 3）、波形蛋白（VIME）、α-烯醇化酶（ENO 1）、磷酸甘油酸脱氢酶和硒结合蛋白1（SBP 1）。60%（n=24/40）不孕症和POF血清中重组ALDH 1A 1、SBP 1或烯醇化酶阳性。AOA阳性血清中有80.7%（n=21/26）的血清对三种抗原中的一种或多种有抗体，而AOA阴性血清中只有7%（n=1/14）的血清对重组蛋白有抗体。ALDH 1A 1和SBP 1是与不孕症和POF相关的卵巢自身免疫所特有的，并可能为识别卵巢自身免疫患者的特异性检测提供基础。
To identify ovarian autoantigens associated with ovarian autoantibodies. Hypothesis generating prospective study. Urban infertility referral centers and academic research institution. 74 patients with infertility, 19 patients with premature ovarian failure, 16 healthy control women. None. Identification of autoantigens. In order to identify major antigens for ovarian autoimmunity, 74 sera from women with unexplained infertility were screened for ovarian autoantibodies (AOA) by immunoassay and one-dimensional Western blot. The majority of sera had immuno-reactions at 50-56kDa. Six representative positive infertility sera were used to identify antigens between 40-60kD by two-dimensional Western blot and mass spectrometry. Antigens included aldehyde (retinal) dehydrogenases (ALDH1A1, ALDH1A2, ALDH7A1), protein disulfide-isomerase A3 (PDIA3), vimentin (VIME), α-enolase (ENO1), phosphoglycerate dehydrogenase and selenium binding protein 1 (SBP1). 60% (n=24/40) of infertility and POF sera were positive for recombinant ALDH1A1, SBP1 or enolase. 80.7% (n=21/26) of AOA positive sera had antibodies to one or more of the three antigens, while only 7% (n=1/14) of AOA negative sera had antibodies to recombinant proteins. ALDH1A1 and SBP1 are unique to ovarian autoimmunity associated with infertility and POF, and may provide the basis for specific tests to identify patients with ovarian autoimmunity.
登录
查看更多内容
影响因子：
158.5
作者：
Arbuckle, MR;McClain, MT;Harley, JB
通讯作者：
Harley, JB
影响因子：
3.5
作者：
Hattori, MA;Takesue, K;Fujihara, N
通讯作者：
Fujihara, N
影响因子：
2.9
作者：
Forges, Thierry;Monnier-Barbarino, Patricia;Bene, Marie-Christine
通讯作者：
Bene, Marie-Christine
影响因子：
4.9
作者：
El-Gabalawy HS;Wilkins JA
通讯作者：
Wilkins JA
DOI：
10.1186/1477-7827-1-27
发表时间：
2003-02-14
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Calvert, Meredith E;Digilio, Laura C;Coonrod, Scott A
通讯作者：
Coonrod, Scott A