Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino
Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino
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DOI：
10.1007/s00240-007-0114-4
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发表时间：
2007-12-01
影响因子：
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通讯作者：
Suzuki, Koji
中科院分区：
文献类型：
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作者：
Moriyama, Manabu T.;Miyazawa, Katsuhito;Suzuki, Koji
Urocalun（（R））是一种从栎属柳/狭叶栎属（Quercus stenophylla Makino）的提取物（QS提取物）制备的草药，自1969年以来在日本已在临床上用于治疗尿石症。本研究观察了QS提取物对琥珀酸诱导的细胞损伤和NADPH诱导的损伤细胞超氧阴离子（O-2（-））产生的影响。通过3-（4，5-二甲基-2-噻唑）-2，5-二苯基四唑溴化物的线粒体还原和乳酸脱氢酶渗漏到细胞外液中来评估草酸盐诱导的细胞损伤。当NRK-52 E细胞暴露于草酸盐24小时后，QS提取物以剂量依赖性方式防止损伤。此外，QS提取物抑制NADPH诱导的O-2（-）生产的增加，或NADPH氧化酶活性，在草酸暴露损伤的细胞匀浆。这些结果表明，减少油酸诱导的O-2（-）的生产有助于QS提取物的细胞保护作用。
Urocalun((R)), a herbal medicine prepared from an extract of Quercus salicina Blume/Quercus stenophylla Makino (QS extract), has been clinically used for the treatment of urolithiasis in Japan since 1969. In the present study, the effects of QS extract on oxalate-induced cell injury and NADPH-induced superoxide anion (O-2(-)) production in the injured cells were investigated. Oxalate-induced cell injury was assessed by mitochondrial reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide and leakage of lactate dehydrogenase into the extracellular fluid. When NRK-52E cells were injured by exposure to oxalate for 24 h, QS extract prevented the injury in a dose-dependent manner. In addition, QS extract suppressed the increase in NADPH-induced O-2(-) production, or NADPH oxidase activity, in the homogenate of cells injured by oxalate exposure. These findings suggest that the reduction in oxalate-induced O-2(-) production contributes to the cytoprotective effect of QS extract.