Performance of a fast fiber based UV/Vis multiwavelength detector for the analytical ultracentrifuge
Performance of a fast fiber based UV/Vis multiwavelength detector for the analytical ultracentrifuge
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DOI：
10.1007/s00396-007-1815-5
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发表时间：
2008-02-01
影响因子：
2.4
通讯作者：
Coelfen, Helmut
中科院分区：
文献类型：
--
作者：
Strauss, Holger M.;Karabudak, Engin;Coelfen, Helmut
光学设置和性能的原型UV/维斯多波长分析超吸收（MWL-AUC）的描述和比较，市售的Optima XL-A从贝克曼库尔特。对MWL-AUC的光路进行了轻微修改。在波长精度和径向分辨率方面，发现新的MWL-AUC与现有的XL-A相当。吸光度准确度取决于检测波长处可用的光强度以及数据的固有噪声。单次闪光的测量值对于MWL-AUC而言噪声更大，可能是由于当前设计中缺少闪光间归一化。然而，波长和扫描平均的可能性可以补偿这一点，并且仍然提供比XL-A快得多的扫描速率。根据这些发现，对现有设计提出了一些进一步的改进建议。
The optical setup and the performance of a prototype UV/Vis multiwavelength analytical ultracentrifuge (MWL-AUC) is described and compared to the commercially available Optima XL-A from Beckman Coulter. Slight modifications have been made to the optical path of the MWL-AUC. With respect to wavelength accuracy and radial resolution, the new MWL-AUC is found to be comparable to the existing XL-A. Absorbance accuracy is dependent on the light intensity available at the detection wavelength as well as the intrinsic noise of the data. Measurements from single flashes of light are more noisy for the MWL-AUC, potentially due to the absence of flash-to-flash normalization in the current design. However, the possibility of both wavelength and scan averaging can compensate for this and still give much faster scan rates than the XL-A. Some further improvements of the existing design are suggested based on these findings.