Fecal glucocorticoids: A noninvasive method of measuring adrenal activity in wild and captive rodents
Fecal glucocorticoids: A noninvasive method of measuring adrenal activity in wild and captive rodents
复制标题
DOI：
10.1086/316721
复制
发表时间：
2000-01-01
影响因子：
1.6
通讯作者：
Austad, SN
中科院分区：
文献类型：
--
作者：
Harper, JM;Austad, SN
为了确定粪便中皮质类固醇浓度在实验室和野外条件下作为慢性应激指标的有效性，我们从生物化学和生理学的角度验证了三种啮齿动物--家鼠(Mus Musculus)、鹿鼠(Permyscus Manulatus)和红背田鼠(Cleys Onomys Gapperi)的皮质类固醇放射免疫分析方法。生化验证表明，该方法准确、准确地测定了粪便中的皮质类固醇浓度。生理学验证表明，该检测方法足够灵敏，可以检测到与(A)动物的短暂接触和出血，(B)慢性热量限制，(C)暴露在新环境中，以及(D)暴露在新的寒冷环境中有关的应激。我们的结果表明，粪便测量反映了这些动物在排便前大约6-12小时所经历的压力水平。因此，给出了一个明智的诱捕和诱捕监测方案，这种测试在测量动物在野外实际存在的应激水平方面具有相当大的实用价值。
To determine the utility of fecal corticosteroid concentration as a measure of chronic stress under laboratory and field conditions, we biochemically and physiologically validated a radioimmunoassay for corticosteroids in three rodent species, house mice (Mus musculus), deer mice (Peromyscus maniculatus), and red-back voles (Clethrionomys gapperi). The biochemical validations demonstrated that the assay accurately and precisely measured corticosteroid concentration in the feces. The physiological validation indicated that the assay was sensitive enough to detect the stress associated with (a) brief handling and bleeding of animals, (b) chronic caloric restriction, (c) exposure to a novel environment, and (d) exposure to a novel cold environment. Our results suggest that fecal measurements reflect stress levels experienced by these animals approximately 6-12 h before defecation. Therefore, given a judicious trapping and trap-monitoring protocol, this assay has considerable utility for measuring the stress levels at which animals actually exist in the field.