Distribution of antibiotic resistance genes in Bosten Lake, Xinjiang, China.
Distribution of antibiotic resistance genes in Bosten Lake, Xinjiang, China.
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DOI：
10.2166/wst.2014.321
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发表时间：
2014-09
期刊：
影响因子：
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通讯作者：
Ting Zhou;Jianjiang Lu;Yanbin Tong;Shanman Li;Xiaolong Wang
中科院分区：
文献类型：
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作者：
Ting Zhou;Jianjiang Lu;Yanbin Tong;Shanman Li;Xiaolong Wang
对新疆博斯腾湖17个水样中抗生素耐药基因和耐药菌进行了定量检测。采用异养平板计数法检测磺胺类和四环素类耐药菌，平均浓度分别为2.50× 105和4.63× 103 CFU/mL。磺胺类（sul 1，sul 2）和四环素类（tetM，tetO和tetW）耐药基因的检测结果表明，除tetO基因外，所有样品均获得了其它ARG基因。对获得的4个ARG进行了进一步的定量分析，结果表明磺胺类药物耐药基因普遍存在。sul 1/16 s-rRNA相对丰度为2.81×10 ~（-5）~ 3.33×10 ~（-3），sul 2/16 s-rRNA相对丰度为1.04×10 ~（-5）~ 3.80×10 ~（-3）。泰特基因tetM/16 s-rRNA和tetW 16 s-rRNA的相对浓度分别为1.18×10（-5）~ 2.46×10（-4）和1.58×10（-6）~ 4.19×10（-4）。ARGs之间的浓度差异可能与每个基因的不同特性有关。本研究证实博斯腾湖受到ARGs和耐药菌的影响，成为ARGs和耐药菌转移的重要水库。
The occurrence of antibiotic resistance genes (ARGs) and resistant bacteria was quantified in 17 water samples collected across Bosten Lake, Xinjiang, China. The heterotrophic plate count method was used to detect the levels of sulfonamide- and tetracycline-resistant bacteria, which have mean concentrations of 2.50×10(5) and 4.63×10(3) CFU/mL, respectively. The resistance genes of sulfonamide (sul1, sul2) and tetracycline (tetM, tetO and tetW) were detected, and results showed that all other ARGs except the tetO gene were obtained from all samples. Four of the obtained ARGs were further quantified, and results showed that the sulfonamide resistance genes were prevalent. The relative abundance was in the range of 2.81×10(-5) to 3.33×10(-3) for the sul1/16s-rRNA and 1.04×10(-5) to 3.80×10(-3) for the sul2/16s-rRNA. For the tet genes, the relative concentrations of tetM/16s-rRNA and tetW16s-rRNA ranged from 1.18×10(-5) to 2.46×10(-4) and 1.58×10(-6) to 4.19×10(-4), respectively. The concentration divergence among ARGs may be related to the different characteristics of each gene. This study validated that Bosten Lake was affected by ARGs and resistant bacteria, thus turning the lake into an important reservoir for the transfer of ARGs and resistant bacteria.