Development and evaluation of EST-SSR markers in Sorbus pohuashanensis (Hance) Hedl. and their application to other Sorbus species
Development and evaluation of EST-SSR markers in Sorbus pohuashanensis (Hance) Hedl. and their application to other Sorbus species
复制标题
花楸EST-SSR标记的开发与评价。
DOI：
10.1007/s00468-019-01928-0
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发表时间：
2019-11-08
影响因子：
2.3
通讯作者：
Zheng，Jian
中科院分区：
文献类型：
--
作者：
Wu，Yuhan;He，Ruiqing;Zheng，Jian
摘要开发并鉴定了一组新的坡花山sorbus (Hance) hel的EST-SSR多态性标记。并生成了最优抽样策略。摘要茯苓（sorbus pohuashanensis）；它原产于中国北方，因其优美的外观和叶色的季节性变化而备受赞誉。然而，对该物种的分子生物学研究很少。因此，它的开发和利用是有限的。本研究的目的是基于该物种的转录组数据开发表达序列标签-简单序列重复（EST-SSR）标记。共设计167对引物，从最初筛选的55对引物中随机选择24对进行验证。利用山东省昆嵛山（SDKYS）种群样本对其多态性进行了分析。15个EST-SSR标记具有多态性，平均等位基因数（Na）为8.2，多态性信息含量（PIC）为0.6907。30个est - ssr（包括先前正在开发的另外15个est - ssr）源自。pohuashanensis对其他14个sorbuss物种具有较高的可移植性（90%），有9个EST-SSRs适用于sorbuss物种。基于9个EST-SSRs，采用UPGMA聚类树法将esorbusspecies划分为2个主要聚类，并进行主坐标分析（PCoA）验证。抽样策略表明，从s。坡花山可以代表其95%的遗传多样性水平。这些研究结果将为进一步研究黄花苜蓿的遗传多样性和种质资源提供有力的理论依据。坡花山及其近缘种。
Key messageWe developed and identified a new set of polymorphic EST-SSR markers inSorbus pohuashanensis(Hance) Hedl. and generated an optimal sampling strategy.AbstractSorbus pohuashanensis(Hance) Hedl. is indigenous to northern China and prized for its graceful appearance and seasonal variations in foliage color. However, only a few molecular biology studies have been conducted on this species. Therefore, its development and increased utilization are limited. The objective of the present study was to develop expressed sequence tag-simple sequence repeat (EST-SSR) markers based on the transcriptome data of this species. A total of 167 primer pairs were designed and 24 were randomly selected from the 55 initially screened for validation. Their polymorphism was assessed using samples of the Mount Kunyu in Shandong Province (SDKYS) population. Fifteen EST-SSR markers were identified as polymorphic, with a mean number of alleles (Na) and a polymorphism information content (PIC) of 8.2 and 0.6907, respectively. Thirty EST-SSRs (including another 15 EST-SSRs being previously developed) derived fromS. pohuashanensishad high transferability (90%) to another 14Sorbusspecies, and there were nine EST-SSRs applicable toSorbusspecies tested. TheseSorbusspecies were divided into two main clusters by an unweighted pair-group method with the arithmetic mean (UPGMA) clustering tree based on the nine EST-SSRs and was validated by principal coordinate analysis (PCoA). The sampling strategy showed that 25 randomly selected individuals from a population ofS. pohuashanensiscould represent 95% of its genetic diversity level. In all, these findings would provide a powerful theoretical basis for future studies on the genetic diversity and germplasm resources ofS. pohuashanensisand its related species.