Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations.
Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations.
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DOI：
10.1038/s41467-018-06318-7
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发表时间：
2018-10-22
影响因子：
16.6
通讯作者：
McGilvray ID
中科院分区：
文献类型：
--
作者：
MacParland SA;Liu JC;Ma XZ;Innes BT;Bartczak AM;Gage BK;Manuel J;Khuu N;Echeverri J;Linares I;Gupta R;Cheng ML;Liu LY;Camat D;Chung SW;Seliga RK;Shao Z;Lee E;Ogawa S;Ogawa M;Wilson MD;Fish JE;Selzner M;Ghanekar A;Grant D;Greig P;Sapisochin G;Selzner N;Winegarden N;Adeyi O;Keller G;Bader GD;McGilvray ID
肝脏是人体最大的实体器官，对代谢和免疫功能至关重要。然而，人们对构成人类肝脏及其免疫微环境的细胞知之甚少。在这里，我们报告了一个地图的细胞景观的人类肝脏使用单细胞RNA测序。我们提供了8444个实质和非实质细胞的转录谱，这些细胞是从5个人肝脏的新鲜肝组织中分离获得的。使用基因表达模式，流式细胞术和免疫组化检查，我们确定了20个离散的细胞群的肝细胞，内皮细胞，胆管细胞，肝星状细胞，B细胞，传统和非传统的T细胞，NK样细胞，和不同的肝内单核细胞/巨噬细胞群体。总之，我们的研究以单细胞分辨率呈现了人类肝脏的全面视图，概述了肝脏中驻留细胞的特征，特别是提供了人类肝脏免疫微环境的地图。单细胞RNA测序技术的发展有助于推进我们对组织生物学的理解。在这里，MacParland等人对人类肝脏样本进行了单细胞RNA测序，并鉴定了可能在肝脏疾病中发挥特定作用的肝内巨噬细胞的不同群体。
The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RNA sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal cells obtained from the fractionation of fresh hepatic tissue from five human livers. Using gene expression patterns, flow cytometry, and immunohistochemical examinations, we identify 20 discrete cell populations of hepatocytes, endothelial cells, cholangiocytes, hepatic stellate cells, B cells, conventional and non-conventional T cells, NK-like cells, and distinct intrahepatic monocyte/macrophage populations. Together, our study presents a comprehensive view of the human liver at single-cell resolution that outlines the characteristics of resident cells in the liver, and in particular provides a map of the human hepatic immune microenvironment. The development of single cell RNA sequencing technologies has been instrumental in advancing our understanding of tissue biology. Here, MacParland et al. performed single cell RNA sequencing of human liver samples, and identify distinct populations of intrahepatic macrophages that may play specific roles in liver disease.
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影响因子：
64.8
作者：
Halpern KB;Shenhav R;Matcovitch-Natan O;Toth B;Lemze D;Golan M;Massasa EE;Baydatch S;Landen S;Moor AE;Brandis A;Giladi A;Avihail AS;David E;Amit I;Itzkovitz S
通讯作者：
Itzkovitz S
影响因子：
13.5
作者：
DeLeve, Laurie D.
通讯作者：
DeLeve, Laurie D.
影响因子：
64.5
作者：
Enge M;Arda HE;Mignardi M;Beausang J;Bottino R;Kim SK;Quake SR
通讯作者：
Quake SR
影响因子：
9.3
作者：
Baron M;Veres A;Wolock SL;Faust AL;Gaujoux R;Vetere A;Ryu JH;Wagner BK;Shen-Orr SS;Klein AM;Melton DA;Yanai I
通讯作者：
Yanai I
影响因子：
11.1
作者：
Baker D;Marta M;Pryce G;Giovannoni G;Schmierer K
通讯作者：
Schmierer K