Gene isolation with cDNA probes from identified Aplysia neurons: neuropeptide modulators of cardiovascular physiology.
Gene isolation with cDNA probes from identified Aplysia neurons: neuropeptide modulators of cardiovascular physiology.
复制标题
使用来自已鉴定的海兔神经元的 cDNA 探针进行基因分离：心血管生理学的神经肽调节剂。
DOI：
10.1016/0092-8674(83)90206-4
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发表时间：
1983
期刊：
影响因子：
64.5
通讯作者：
Scheller,RH
中科院分区：
文献类型：
--
作者：
Nambu,JR;Taussig,R;Mahon,AC;Scheller,RH
The Aplysia abdominal ganglion neurons, R3-R14, modulate cardiovascular activity. In vitro translations of poly (A)+ RNA from these cells suggest that they contain a prevalent mRNA encoding a 14 kd protein. Utilizing differential screening techniques with 32P-labeled cDNA synthesized from the poly (A)+ RNA of identified neurons, we isolated the corresponding gene. The Aplysia haploid genome contains a single copy of this sequence, which is interrupted by two large introns and spans-7 kb of genomic DNA. The R3-R14 neurons specifically express this gene, resulting in the synthesis of a 1. 25 kb mRNA not found in other abdominal ganglion cells or in the head ganglia. The gene was shown to encode a 13.5 kd precursor, which is proteolytically cleaved into at least three peptides with molecular weights of 5.0, 3.3, and 1.3 kd. These peptides and glycine are thought to act as chemical messengers in the central nervous system and peripherally.
