Isolation of Spiroplasma sp from an Ixodes tick
Isolation of Spiroplasma sp from an Ixodes tick
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DOI：
10.1016/j.ijmm.2006.01.012
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发表时间：
2006-05-01
影响因子：
4.1
通讯作者：
Theegarten, Dirk
中科院分区：
文献类型：
--
作者：
Henning, Klaus;Greiner-Fischer, Susanne;Theegarten, Dirk
螺旋体是一种螺旋状的支原体，存在于植物和节肢动物中，也存在于壁虱中。一些螺旋体物种被认为是脊椎动物的潜在病原体。在对北莱茵-威斯特伐利亚Q热病的研究中，可以从硬蜱的池中分离出一种细胞内生长的微生物。该病原体在细胞质空泡中复制，与伯氏柯克斯体相似。用柯克斯体、埃立克体或衣原体特异的引物进行聚合酶链式反应，结果表明Z/16菌剂与这些细菌不同。与柯氏或衣原体相比，该试剂不能按Gimenez法染色。此外，电子显微镜还提供了Z/16菌株不同于柯西拉克链霉菌和衣原体的证据。对16S核糖体RNA基因序列的测定表明，分离株Z/16可归入螺旋体属。据我们所知，这是使用哺乳动物细胞系分离出螺旋体株的第一份报告。该生物体的致病潜力需要进一步研究。(C)2006年爱思唯尔股份有限公司。版权所有。
Spiroplasmas are helical mycoplasmas which are found in plants and arthropods, also in ticks. Some Spiroplasma species are incriminated as potential pathogens for vertebrates. During a study on Q fever in North Rhine-Westphalia, an intracellularly growing microorganism could be isolated from a pool of Ixodes ticks. The agent replicated within cytoplasmic vacuoles similar to those of Coxiella burnetii. PCR using coxiellae-, ehrlichiae- or chlamydiae-specific primers showed that agent Z/16 was distinct from these bacteria. In contrast to coxiellae or chlamydiae, the agent could not be stained according to the method of Gimenez. Also electron microscopy provided evidence that the isolate Z/16 is different from coxiellac and chlamydiae. Determination of 16S ribosomal RNA gene sequences provided evidence that the isolate Z/16 can be classified as Spiroplasma sp. To our knowledge, this is the first report of an isolation of a Spiroplasma strain using a mammalian cell line. The pathogenic potential of the organism needs further investigation. (c) 2006 Elsevier GmbH. All rights reserved.