Th2 cells mediate IL-4-dependent local tissue inflammation.
Th2 cells mediate IL-4-dependent local tissue inflammation.
复制标题
Th2 细胞介导 IL-4 依赖性局部组织炎症。
DOI：
10.4049/jimmunol.150.12.5576
复制
发表时间：
1993
影响因子：
4.4
通讯作者：
Conrad Hauser
中科院分区：
文献类型：
--
作者：
K. M. Müller;F. Jaunin;Isabelle Masouyé;J. Saurat;Conrad Hauser
我们研究了体外短期培养获得的多克隆小鼠Th1和Th2细胞在体内是否具有介导组织炎症的能力。用单克隆抗体（mAb）对TCR/CD3复合物进行脉冲，并将细胞注射到幼稚的同基因受体小鼠的脚垫或耳朵中。Th1诱导迟发性肿胀在24 ~ 48 h达到高峰，持续50 ~ 5天。th2诱导的肿胀在6小时达到顶峰，持续时间<或= 48小时。在胸腺裸小鼠中也观察到类似的反应。Th1和Th2细胞的病变在6小时时均以嗜中性粒细胞浸润为主，在48小时时以单核细胞浸润为主。Th2的炎症反应可被环孢素a、对IL-4的单抗或可溶性il - 4r阻断。对IL-4的需求是早期和短暂的。四个同种异体反应性短期Th2克隆诱导异体受体肿胀。在两个长期的Th2克隆中也观察到单抗和il -4依赖的肿胀反应。我们的研究结果表明Th2细胞介导il -4依赖的组织炎症，并加强了Th2细胞在一些T细胞依赖的免疫反应中发挥重要作用的概念，并且可能在过敏性疾病如特应性皮炎和过敏性哮喘中发挥重要作用。
We investigated whether polyclonal murine Th1 and Th2 cells obtained after short term culture in vitro were capable of mediating tissue inflammation in vivo. Th cells were pulsed with mAb to the TCR/CD3 complex and injected into the footpads or ears of naive syngeneic recipient mice. Th1 induced delayed swelling that peaked at 24 to 48 h and lasted > or = 5 days. Th2-induced swelling peaked at 6 h and lasted < or = 48 h. Similar responses were also observed in athymic nude mice. Lesions with both Th1 and Th2 cells contained a predominant neutrophilic infiltrate at 6 h, and mainly mononuclear cells at 48 h. The inflammatory response with Th2 was blocked by cyclosporin A, by mAb to IL-4 or by soluble rIL-4R. The requirement for IL-4 was early and transient. Four alloreactive short term Th2 clones induced swelling in allogeneic recipients. mAb- and IL-4-dependent swelling responses were also observed with two long term Th2 clones. Our results demonstrate that Th2 cells mediate IL-4-dependent tissue inflammation, and strengthen the concept that Th2 cells play an important role in some T cell-dependent immune reactions and, possibly, in allergic disorders such as atopic dermatitis and allergic asthma.