Polymorphic minisatellites in the mitochondrial DNAs of Oryza and Brassica
Polymorphic minisatellites in the mitochondrial DNAs of Oryza and Brassica
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DOI：
10.1007/s00294-011-0345-3
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发表时间：
2011-08-01
期刊：
影响因子：
2.5
通讯作者：
Kubo, Tomohiko
中科院分区：
文献类型：
--
作者：
Honma, Yujiro;Yoshida, Yu;Kubo, Tomohiko
与叶绿体DNA相比，被子植物线粒体DNA的多态性分析很少，因为被子植物线粒体DNA中的靶序列很少。小卫星是重复序列的串联阵列，重复单位为10至100 bp，是动物线粒体的常见靶序列，但甜菜是唯一已知的被子植物物种，已进行了这样的分析。由于缺乏信息，尚不确定其他被子植物物种中是否存在多态小卫星。在10种植物线粒体DNA中发现了小卫星样重复序列，大部分位于基因间区，少数位于基因编码区和内含子区。水稻和芸苔属的种质资源中存在丰富的系统信息，因此被选为研究小卫星多态性的模式。对42份稻属材料的PCR分析表明，5个小卫星中有4个存在长度多态性。线粒体单倍型的16个水稻品种的染色体互补（基因组）类型的CC，BBCC和CCDD是相同的，但明显区别于BB基因组的加入，一个结果与双二倍体物种的细胞质供体亲本可能是CC基因组物种的概念是一致的。根据6个微卫星位点的2个多态性，将芸苔属6个主要栽培种的29份材料分为5种线粒体单倍型。芥菜和埃塞俄比亚芥的单倍型分别与芜菁和黑芥的单倍型相同。甘蓝型油菜的单倍型是异质的和独特的，结果与以前的研究一致。
Polymorphic analyses of angiosperm mitochondrial DNA are rare in comparison with chloroplast DNA, because few target sequences in angiosperm mitochondrial DNA are known. Minisatellites, a tandem array of repeated sequences with a repeat unit of 10 to similar to 100 bp, are popular target sequences of animal mitochondria, but Beta vulgaris is the only known angiosperm species for which such an analysis has been conducted. From this lack of information, it was uncertain as to whether polymorphic minisatellites existed in other angiosperm species. Ten plant mitochondrial DNAs were found to contain minisatellite-like repeated sequences, most of which were located in intergenic regions but a few occurred in gene coding and intronic regions. Oryza and Brassica accessions were selected as models for the investigation of minisatellite polymorphism because substantial systematic information existed. PCR analysis of 42 Oryza accessions revealed length polymorphisms in four of the five minisatellites. The mitochondrial haplotypes of the 16 Oryza accessions with chromosomal complement (genome) types of CC, BBCC and CCDD were identical but were clearly distinguished from BB-genome accessions, a result consistent with the notion that the cytoplasmic donor parent of the amphidiploid species might be the CC-genome species. Twenty-nine accessions of six major cultivated species of Brassica were classified into five mitochondrial haplotypes based on two polymorphic minisatellites out of six loci. The haplotypes of Brassica juncea and Brassica carinata accessions were identical to Brassica rapa and Brassica nigra accessions, respectively. The haplotypes of Brassica napus accessions were heterogeneous and unique, results that were consistent with previous studies.