Surface energy effects on osteoblast spatial growth and mineralization
Surface energy effects on osteoblast spatial growth and mineralization
复制标题
DOI：
10.1016/j.biomaterials.2007.12.026
复制
发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Donahue, Henry J.
中科院分区：
文献类型：
--
作者：
Lim, Jung Yul;Shaughnessy, Michael C.;Donahue, Henry J.
虽然短期的表面能对细胞粘附的影响是相对众所周知的，很少有人透露，关于其后期阶段的影响细胞的行为。我们研究了表面能对成骨细胞生长和矿化的影响，使用人胎儿成骨细胞（hFOB）细胞培养等离子体处理的石英（接触角，θ = 0度）和十八烷基三氯硅烷（OTS）处理的石英（θ = 113度）。hFOB细胞在等离子体处理的石英上形成均匀的细胞层，而在OTS处理的石英上培养的细胞产生随机分布的团块状结构，其充满细胞（通过共聚焦显微镜证实）。当在亲水性表面上培养时，hFOB细胞的矿物质沉积在空间上是均匀的。此外，亲水性表面上的细胞表现出增加的矿化面积以及增强的矿物质-基质比（通过傅立叶变换红外光谱法评估），相对于疏水性表面上的细胞。使用其他类型的成骨细胞样细胞（MC 3 T3-E1，MG 63和SAOS-2）的实验显示了或多或少类似的空间生长形态学效应。得出的结论是，亲水性表面诱导均匀的空间成骨细胞生长和矿物质沉积，并提高了成骨细胞的数量（例如，面积）和质量（例如，矿物与基质的比率）相对于疏水表面的矿化。我们的数据表明，表面能对成骨细胞分化的影响，特别是矿化，可能与空间细胞生长的表面能依赖性变化。(C)2008年由Elsevier Ltd.出版
While short-term surface energy effects on cell adhesion are relatively well known, little is revealed as regards its later stage effects on cell behavior. We examined surface energy effects on osteoblastic cell growth and mineralization by using human fetal ostcoblastic (hFOB) cells cultured on plasma-treated quartz (contact angle, theta = 0 degrees) and octadecyltrichlorosilane (OTS)-treated quartz (theta = 113 degrees). hFOB cells formed a homogeneous cell layer on plasma-treated quartz, while those cultured on OTS-treated quartz produced randomly distributed clump-like structures that were filled with cells (confirmed by confocal microscopy). Mineral deposition by hFOB cells was spatially homogeneous when cultured on hydrophilic surfaces. Furthermore, cells on hydrophilic surfaces exhibited increased mineralized area as well as enhanced mineral-to-matrix ratio (assessed by Fourier transform infrared spectroscopy), relative to cells on hydrophobic surfaces. Experiments using other types of osteoblast-like cells (MC3T3-E1, MG63, and SAOS-2) revealed more or less similar effects in spatial growth morphology. It was concluded that hydrophilic surfaces induce homogeneous spatial osteoblastic cell growth and mineral deposition and enhance the quantity (e.g., area) and quality (e.g., mineral-to-matrix ratio) of mineralization relative to hydrophobic surfaces. Our data suggest that surface energy effects on osteoblastic cell differentiation, especially mineralization, may be correlated with surface energy dependent changes in spatial cell growth. (C) 2008 Published by Elsevier Ltd.