Effects of titanium with different micro/nano structures on the ability of osteoblasts to resist oxidative stress
Effects of titanium with different micro/nano structures on the ability of osteoblasts to resist oxidative stress
复制标题
不同微纳结构钛对成骨细胞抗氧化应激能力的影响
DOI：
10.1016/j.msec.2021.111969
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发表时间：
2021-02-18
影响因子：
7.9
通讯作者：
Liu, Jinsong
中科院分区：
文献类型：
--
作者：
Ma, Pingping;Yu, Yonglin;Liu, Jinsong
氧化中间产物在老年人体内的过量积累会显著加速骨降解，并阻碍拓扑钛（Ti）种植体的骨结合。因此，评价各种纳米、微米或微米/纳米复合结构在氧化应激（oxidative stress，OS）微环境下的抗氧化和骨诱导能力具有重要意义。在这项研究中，我们发现，110 nm的二氧化钛纳米管（TNTs）增强纤维连接蛋白（FN）蛋白质的吸附到光滑和粗糙的钛表面不同程度。与Ti和30 nm TNTs（T30）组相比，110 nm TNTs（T110）、微结构/30 nm TNTs（M30）和微结构/110 nm TNTs（M110）上的细胞在OS条件下面积更小，活性氧（ROS）更低，增殖/成骨分化能力更强，但3组间差异无统计学意义。此外，结合我们以前的研究，我们认为T110，M30和M110对OS的抵抗也与FN受体整合素的高表达密切相关。5还是？1.结果表明，微/纳米复合结构（M30和M110）具有与T110相似的抗氧化和成骨能力，为不同拓扑结构钛种植体在老年人中的应用提供了指导。
Excessive accumulation of oxidative intermediates in the elderly significantly aggravates bone degradation and hinders the osseointegration of topological titanium (Ti) implants. Thus, it is of great significance to evaluate the antioxidant and osteoinduction capabilities of various nano, micro or micro/nano-composite structures under oxidative stress (OS) microenvironment. In this study, we discovered that 110 nm titania nanotubes (TNTs) enhanced the adsorption of fibronectin (FN) proteins onto smooth and rough titanium surfaces to varying degrees. Compared with Ti and 30 nm TNTs (T30) groups, cells on 110 nm TNTs (T110), microstructure/30 nm TNTs (M30) and microstructure/110 nm TNTs (M110) had smaller area, lower reactive oxygen species (ROS), and better proliferation/osteogenic differentiation abilities under OS condition, but there was no significant difference among the three groups. In addition, combined with our previous study, we suggested that T110, M30 and M110 resistance to OS was also strongly associated with the high expression of FN-receptor integrin ?5 or ?1. All the findings indicated that the micro/nano-composed structures (M30 & M110) had similar anti-oxidation and osteogenesis abilities to T110, which provided guidance for the application of different titanium implants with different topologies in the elderly.