Expression of MUC2 and MUC3 mRNA in human normal, malignant, and inflammatory intestinal tissues
Expression of MUC2 and MUC3 mRNA in human normal, malignant, and inflammatory intestinal tissues
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DOI：
10.1177/44.10.8813081
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发表时间：
1996-10-01
影响因子：
3.2
通讯作者：
Itzkowitz, SH
中科院分区：
文献类型：
--
作者：
Weiss, AA;Babyatsky, MW;Itzkowitz, SH
MUC2和MUC3是在人肠中表达的主要粘蛋白基因。使用RNA探针原位杂交，我们研究了MUC2和MUC3 mRNA的细胞分布在正常，恶性和炎症的人肠组织。在正常小肠和结肠。MUC2 mRNA仅在杯状细胞中表达，并在整个粘膜高度上表达。MUC3 mRNA由杯状细胞和柱状细胞表达，但仅限于小肠的绒毛隔室和结肠的表面上皮。MUC2和MUC3 mRNA的表达在低、中、高分化结肠癌中均显著降低，但在粘液性结肠癌中保持不变。在溃疡性结肠炎和克罗恩氏结肠炎组织中，MUC2和MUC3 mRNA表达显示正常模式，无论粘膜是否表现出活动性或静止性炎症。这些发现表明MUC2是杯状细胞特异性的，而MUC3与肠上皮细胞的成熟有关。在结肠癌中，MUC2和MUC3的遗传调节根据肿瘤的组织学类型而不同。炎症性肠病中MC 2和MUC3 mRNA的组成性表达表明，这些基因可能是维持炎症期间正常上皮细胞功能所必需的。
MUC2 and MUC3 are prominent mucin genes expressed in the human intestine. Using in situ hybridization with RNA probes, we examined the cellular distribution of MUC2 and MUC3 mRNA in normal, malignant, and inflammatory human intestinal tissues. In normal small intestine and colon. MUC2 mRNA was expressed exclusively in goblet cells and occurred throughout the entire height of the mucosa. MUC3 mRNA was expressed by goblet and columnar cells but was restricted to the villous compartment of the small intestine and the surface epithelium of the colon. Expression of MUC2 and MUC3 mRNA were both markedly decreased in poorly, moderately, and well-differentiated colon cancers but were preserved in mucinous colon cancers. In ulcerative colitis and Crohn's colitis tissues, MUC2 and MUC3 mRNA expression displayed a normal pattern regardless of whether the mucosa manifested active or quiescent inflammation. These findings indicate that MUC2 is goblet cell-specific, whereas MUC3 is related to maturation of intestinal epithelial cells. In colon cancers, the genetic regulation of MUC2 and MUC3 is different depending on the histological type of tumor. The constitutive expression of MC2 and MUC3 mRNA in inflammatory bowel diseases suggests that these genes may be necessary for maintenance of normal epithelial cell function during inflammation.