Conserved microRNA mediates heating tolerance in germ cells versus surrounding somatic cells
Conserved microRNA mediates heating tolerance in germ cells versus surrounding somatic cells
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保守的 microRNA 介导生殖细胞与周围体细胞的耐热性
DOI：
10.1080/15476286.2019.1639311
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发表时间：
2019-07
期刊：
影响因子：
4.1
通讯作者：
Zeng Shenming
中科院分区：
文献类型：
--
作者：
Wu Yi;Wang Yingzheng;Liu Qiang;Zhu Lihua Julie;Gao Hui;Cui Maosheng;Liu Jinghao;Zhao Pengju;LiU Jianfeng;Chen Lei;Wang Jinyong;Zeng Wenxian;Woodruf Teresa K.;Zeng Shenming
哺乳动物的生育力在夏季的高温暴露期间降低，但在秋季温度再次降低时恢复。然而，这一现象背后的机制仍不清楚。我们研究了生殖细胞及其周围体细胞的热应激耐受性，发现在培养的猪颗粒细胞（GC）中，microRNA ssc-ca-1在热应激后上调，但在血清饥饿的GC中则没有。Ssc-ca-1通过其3′-和5′-UTR抑制热休克蛋白70（Hsp 70）的表达。虽然热休克蛋白70 mRNA的转录诱导GC在体内暴露于热在夏季，ssc-ca-1抑制热休克蛋白70的表达。在卵巢培养中，热应激诱导的Hsp 70表达在原始卵泡中，但在生长卵泡中没有; ssc-ca-1表达在原始卵泡中没有变化，但在生长卵泡中增加。结论：ssc-ca-1在睾丸细胞中存在，其功能与卵巢细胞相同。热休克蛋白70在睾丸支持细胞和精原干细胞中的表达差异。这一机制与世界上受全球气候变化引起的热应激影响的部分地区的哺乳动物生育能力有关。
ABSTRACT Mammalian fertility is reduced during heat exposure in the summer, but is regained as temperatures decrease in the autumn again. However, the mechanism underlying the phenomenon remains unknown. We investigated heat stress tolerance of germ cells and their surrounding somatic cells, and discovered that microRNA ssc-ca-1 was upregulated after heat stress in cultured porcine granulosa cells (GCs), but not in serum-starved GCs. Ssc-ca-1 inhibited heat shock protein 70 (Hsp70) expression through its 3′- and 5′-UTRs. Although Hsp70 mRNA transcription was induced in GCs by in vivo exposure to heat in the summer, ssc-ca-1 inhibited Hsp70 expression. In ovarian cultures, heat stress-induced Hsp70 expression in primordial but not in growing follicles; ssc-ca-1 expression did not change in primordial follicles, but increased in growing follicles. Consistently, ssc-ca-1 was present in testicular cells and exhibited the same function as in ovarian cells. It modulated the different Hsp70 expression between spermatogonial stem cells and Sertoli cells after scrotal heat stress. This mechanism is of relevance to mammalian fertility in parts of the world dominated by heat stress associated with global climate change.
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DOI：
10.1074/jbc.m111.293274
发表时间：
2012-01
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
Xian-long Wang;Yi Wu;L. Tan;Zhen Tian;Jing-hao Liu;De-Sheng Zhu;S. Zeng
通讯作者：
Xian-long Wang;Yi Wu;L. Tan;Zhen Tian;Jing-hao Liu;De-Sheng Zhu;S. Zeng
DOI：
10.1152/ajpheart.00233.2008
发表时间：
2008-12-01
影响因子：
4.8
作者：
Asada, Satoshi;Takahashi, Tomosaburo;Oh, Hidemasa
通讯作者：
Oh, Hidemasa
影响因子：
3.7
作者：
Ting Wang;Qiujing Yu;Juan Chen;Bo Deng;Lihua Qian;Y. Le
通讯作者：
Ting Wang;Qiujing Yu;Juan Chen;Bo Deng;Lihua Qian;Y. Le
影响因子：
4.8
作者：
Li, CY;Lee, JS;Seo, JS
通讯作者：
Seo, JS
影响因子：
4.8
作者：
Stankiewicz, AR;Lachapelle, G;Mosser, DD
通讯作者：
Mosser, DD