INVIVO P-31 NMR-STUDY OF THE METABOLISM OF MURINE MAMMARY 16/C ADENOCARCINOMA AND ITS RESPONSE TO CHEMOTHERAPY, X-RADIATION, AND HYPERTHERMIA
INVIVO P-31 NMR-STUDY OF THE METABOLISM OF MURINE MAMMARY 16/C ADENOCARCINOMA AND ITS RESPONSE TO CHEMOTHERAPY, X-RADIATION, AND HYPERTHERMIA
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DOI：
10.1073/pnas.80.2.334
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
GLICKSON, JD
中科院分区：
文献类型：
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作者：
EVANOCHKO, WT;NG, TC;GLICKSON, JD
具有表面线圈的 31P NMR 波谱已用于监测 C3H/He 小鼠皮下植入的乳腺癌 16/C 腺癌体内的磷酸盐代谢。在未经治疗的肿瘤生长期间以及用阿霉素、热疗和 X 射线治疗后对该模型肿瘤进行了研究。乳腺 16/C 肿瘤表现出 Gompertzian 生长模式。高能磷酸代谢物（磷酸肌酸和 ATP）的水平随着肿瘤质量的增加而下降。 Pi 水平随之增加，肿瘤表观 pH 值降低。这些光谱变化似乎反映了伴随肿瘤生长的肿瘤血管化的变化，肿瘤逐渐变得更加缺氧。该肿瘤对阿霉素化疗的部分反应反映在磷酸肌酸共振小幅但可测量的增加、Pi 降低以及肿瘤内 pH 值恢复至中性。热疗导致 31 P NMR 谱逐渐转变为死亡肿瘤的谱（高水平的 Pi、少量的残留磷酸糖和吡啶二核苷酸以及酸性 pH）。 X 射线照射（14.0 Gy）导致磷酸肌酸峰在治疗后 15 分钟内消失。随后，这种共振又恢复到超过治疗前的水平。随着肿瘤消退，其光谱反映了有氧代谢组织的特征（高水平的磷酸肌酸和 ATP 以及低水平的 Pi 和糖磷酸盐）。
31P NMR spectroscopy with surface coils has been used to monitor,in vivo, the phosphate metabolism of subcutaneously implanted mammary 16/C adenocarcinoma in C3H/He mice. This model tumor was studied during untreated tumor growth and after treatment with adriamycin, hyperthermia, and x-radiation. The mammary 16/C tumor exhibited a Gompertzian growth pattern. Levels of high-energy phosphate metabolites—phosphocreatine and ATP—decreased with increases in tumor mass. There was a concomitant increase in the level of Piand a decrease in the apparent pH of the tumor. These spectral changes appear to reflect changes in tumor vascularization that accompany tumor growth, the tumor becoming progressively more hypoxic. Partial response of this tumor to chemotherapy with adriamycin was reflected in a small but measurable increase in the phosphocreatine resonance, a decrease in Pi, and a return of the intra-tumor pH to neutral. Hyperthermia resulted in progressive conversion of the31P NMR spectrum to that of a dead tumor (high levels of Pi, small levels of residual sugar phosphates and pyridine dinucleotides, and acidic pH). X-irradiation (14.0 Gy) led to disappearance of the phosphocreatine peak within 15 min of treatment. Subsequently, this resonance grew back beyond its pretreatment level. As the tumor receded, its spectrum reflected the characteristics of aerobically metabolizing tissue (high levels of phosphocreatine and ATP and low levels of Piand sugar phosphates).