2 TYPES OF CHOLINERGIC INNERVATION IN CORTEX, ONE CO-LOCALIZED WITH VASOACTIVE INTESTINAL POLYPEPTIDE
2 TYPES OF CHOLINERGIC INNERVATION IN CORTEX, ONE CO-LOCALIZED WITH VASOACTIVE INTESTINAL POLYPEPTIDE
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DOI：
10.1038/309153a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
BAUGHMAN, RW
中科院分区：
文献类型：
--
作者：
ECKENSTEIN, F;BAUGHMAN, RW
哺乳动物大脑皮层中胆碱能神经元细胞体的存在长期以来一直是争议的主题（见参考文献1）。然而，最近，一种特异性的胆碱能标志物，乙酰胆碱合成酶，胆碱乙酰转移酶（ChAT， E.C.2.3.1.6），通过免疫组织化学方法被证明存在于大鼠皮层的双极神经元中2,3。在这里，我们发现至少80%的这些内在胆碱能神经元也含有血管活性肠多肽（VIP）的免疫反应性，VIP是一种存在于皮质神经元亚群中的神经活性肽。另一方面，我们发现基底前脑中的chat阳性细胞（皮层胆碱能神经支配的另一个主要来源）不含可检测到的vip免疫反应性。此外，我们通过光镜和电镜观察到，皮层中一些VIP阳性结构和一些chat阳性结构与血管密切相关。
The existence of cholinergic neuronal cell bodies in mammalian cerebral cortex was long the subject of much controversy (see ref. 1 for review). Recently, however, a specific cholinergic marker, the acetylcholine synthesizing enzyme, choline acetyltransferase (ChAT, E.C.2.3.1.6), was demonstrated by immunohistochemical methods to be present in bipolar neurones in rat cortex2,3. Here we show that at least 80% of these intrinsic cholinergic neurones also contain immunoreactivity for vasoactive intestinal polypeptide (VIP), a neuroactive peptde found to be present in a subpopulation of cortical neurones4. On the other hand, we find that the ChAT-positive cells in the basal forebrain, which are another major source of cholinergic innervation of the cortex5–8, contain no detectable VIP-immunoreactivity. In addition, we have observed by both light and electron microscopy that some VIP- and some ChAT-positive structures in cortex are closely associated with blood vessels.