“Out‐of‐the‐box” Granular Gel Bath Based on Cationic Polyvinyl Alcohol Microgels for Embedded Extrusion Printing
“Out‐of‐the‐box” Granular Gel Bath Based on Cationic Polyvinyl Alcohol Microgels for Embedded Extrusion Printing
复制标题
用于嵌入式挤出印刷的基于阳离子聚乙烯醇微凝胶的“开箱即用”颗粒凝胶浴
DOI：
10.1002/marc.202300025
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发表时间：
2023
影响因子：
4.6
通讯作者：
Matsusaki Michiya
中科院分区：
文献类型：
--
作者：
Zeng Jinfeng;Xie Zhengtian;Dekishima Yasumasa;Kuwagaki Setsuka;Sakai Norihito;Matsusaki Michiya
嵌入式挤出打印为用活细胞制造复杂的基于水凝胶的生物结构提供了一个通用平台。然而，目前支持浴的耗时过程和严格的储存条件阻碍了其商业应用。这项工作报告了一种基于化学交联阳离子聚乙烯醇（PVA）微凝胶的新型“开箱即用”颗粒状支持浴，只需将冻干浴分散在水中即可使用。值得注意的是，通过离子改性，PVA微凝胶产生减小的粒径、均匀的分布和适当的流变特性，有助于高分辨率打印。经过冻干和再分散过程后，离子改性的PVA浴可恢复到其原始状态，粒度、流变性能和打印分辨率保持不变，证明其稳定性和可回收性。冻干有利于颗粒凝胶浴的长期储存和输送，并能够应用“开箱即用”的支持材料，这将大大简化实验程序，避免劳动密集型和耗时的操作，从而加速嵌入式生物打印的广泛商业发展。
Embedded extrusion printing provides a versatile platform for fabricating complex hydrogel‐based biological structures with living cells. However, the time‐consuming process and rigorous storage conditions of current support baths hinder their commercial application. This work reports a novel “out‐of‐the‐box” granular support bath based on chemically crosslinked cationic polyvinyl alcohol (PVA) microgels, which is ready to use by simply dispersing the lyophilized bath in water. Notably, with ionic modification, PVA microgels yield reduced particle size, uniform distribution, and appropriate rheological properties, contributing to high‐resolution printing. Following by the lyophilization and re‐dispersion process, ion‐modified PVA baths recover to its original state, with unchanged particle size, rheological properties, and printing resolution, demonstrating its stability and recoverability. Lyophilization facilitates the long‐term storage and delivery of granular gel baths, and enables the application of “out‐of‐the‐box” support materials, which will greatly simplify experimental procedures, avoid labor‐intensive and time‐consuming operations, thus accelerating the broad commercial development of embedded bioprinting.