Expression of the chondromodulin-I gene in chondrosarcomas
Expression of the chondromodulin-I gene in chondrosarcomas
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DOI：
10.1016/j.canlet.2003.09.015
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发表时间：
2004-02-10
期刊：
影响因子：
9.7
通讯作者：
Toguchida, J
中科院分区：
文献类型：
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作者：
Aoyama, T;Okamoto, T;Toguchida, J
我们研究了软骨调素-I（ChM-I）基因的表达，一个假定的肿瘤抑制基因在软骨肿瘤，定量RT-PCR在15软骨肉瘤（CS）。8个CS中ChM-I基因的表达水平高于关节软骨（阳性病例），而其余7个CS中ChM-I基因的表达水平低于关节软骨（阴性病例）。5例外周型CS均为阳性，且ChM-Ⅰ阳性肿瘤与关节软骨细胞具有相同的软骨相关基因表达谱。另一方面，4例无髓外病变的中央型CS均为阴性，ChM-I阴性肿瘤的甲状旁腺相关肽基因表达水平低于关节软骨细胞，而COL 10A 1基因表达水平高于关节软骨细胞。组织学分级和复发率均与ChM-I基因表达水平无明显相关性。这些结果表明，ChM-Ⅰ基因在CS中的表达与肿瘤的发生没有直接的关系，而是反映了肿瘤发生的部位，因此是肿瘤细胞的前体。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
We investigated the expression of the Chondromodulin-I (ChM-I) gene, a putative tumor suppressor gene in cartilaginous tumors, by quantitative RT-PCR in 15 chondrosarcomas (CSs). Eight CSs expressed the ChM-I gene at the level higher than those in articular cartilage (positive cases), whereas the expression of the ChM-I gene in the remaining seven CSs was lower than those in articular cartilage (negative cases). All of five peripheral CS were positive, and the ChM-I positive tumors shared expression profiles of cartilage-related genes with articular cartilage cells. On the other hand, all of four central CSs without extramedullary lesions were negative, and the ChM-I negative tumors expressed the parathyroid hormone-related peptide gene at the lower level and the COL10A1 genes at the higher level than articular cartilage cells. Neither the histological grade nor the rate of recurrence showed clear association with the level of ChM-I gene expression. These results suggested that the expression of ChM-I gene in CS has no direct role in tumorigenesis but rather reflects the site of tumor development and therefore precursor of tumor cells. (C) 2003 Elsevier Ireland Ltd. All rights reserved.