Detailed ordering of markers localizing to the Xq26-Xqter region of the human X chromosome by the use of an interspecific Mus spretus mouse cross.
Detailed ordering of markers localizing to the Xq26-Xqter region of the human X chromosome by the use of an interspecific Mus spretus mouse cross.
复制标题
通过使用种间小鼠杂交，对位于人类 X 染色体 Xq26-Xqter 区域的标记进行详细排序。
DOI：
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发表时间：
1987
影响因子：
11.1
通讯作者：
J. Cambrou
中科院分区：
文献类型：
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作者：
P. Avner;L. Amar;D. Arnaud;A. Hanauer;J. Cambrou
定位于人X染色体的Xq 26-Xqter区域的五个探针已经使用种间杂交在小鼠X染色体上进行了遗传映射，所述种间杂交涉及小家鼠到位于Tabby（Ta）位点附近的连续区域。系谱和重组分析确定标记顺序为Hprt-FIX-c11-G6 PD-St 14 -1。该连续区域的大小使得X连锁肌营养不良症（mdx）小鼠突变可能映射在该区段内。这反过来表明，小鼠mdx基因座代表杜氏肌营养不良症（DMD）模型的可能性很小。然而，这与这种突变可能对应于人类Emery Dreifuss肌营养不良症的想法是一致的。迄今为止，仅使用有限数量的限制性内切酶，在该种间系统中发现的所有人类交叉反应探针的限制性片段长度多态性频率很高，提示，Mus spretus作图系统在建立人类染色体保守区域的连锁关系和人/鼠杂交中可能具有很大的潜在价值。反应探针是可用的或可以获得的。
Five probes localizing to the Xq26-Xqter region of the human X chromosome have been genetically mapped on the mouse X chromosome using an interspecific cross involving Mus spretus to a contiguous region lying proximally to the Tabby (Ta) locus. Pedigree and recombinational analysis establish the marker order as being Hprt-FIX-c11-G6PD-St14-1. The size of this contiguous region is such that the X-linked muscular dystrophy (mdx) mouse mutation probably maps within this segment. This in turn suggests that it is highly improbable that the mouse mdx locus represents a model for Duchenne muscular dystrophy (DMD). It is, however, compatible with the idea that this mutation may correspond in man to Emery Dreifuss muscular dystrophy. The high frequency of restriction fragment length polymorphisms found in this interspecific system for all the human cross-reacting probes examined up until now, using only a limited number of restriction enzymes, suggests that the Mus spretus mapping system may be of great potential value for establishing the linkage relationships existing in man when conserved chromosomal regions are concerned and human/mouse cross-reacting probes are available or can be obtained.