Species-specific identification of campylobacters by partial 16S rRNA gene sequencing
Species-specific identification of campylobacters by partial 16S rRNA gene sequencing
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DOI：
10.1128/jcm.41.6.2537-2546.2003
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发表时间：
2003-06-01
影响因子：
9.4
通讯作者：
Zechner, EL
中科院分区：
文献类型：
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作者：
Gorkiewicz, G;Feierl, G;Zechner, EL
弯曲杆菌的物种特异性鉴定是有问题的，主要是由于缺乏合适的生化测定和非典型菌株的存在。当表型测试失败时，基于 16S rRNA 基因 (16S rDNA) 的细菌鉴定提供了一种可能的替代方案。因此，我们评估了 16S rDNA 测序用于弯曲杆菌种特异性鉴定的可靠性。使用 135 个表型特征弯曲杆菌菌株（包括该属所有已知的分类群）的近 94% 的完整 16S rRNA 基因进行序列分析。结果表明，16S rDNA 分析能够特异性识别大多数弯曲杆菌属物种。例外是空肠弯曲杆菌、大肠杆菌和非典型拉里杆菌菌株之间缺乏区别，它们具有相同或几乎相同的 16S rDNA 序列。随后，研究了部分 16S rDNA 序列是否足以确定物种身份。序列比对鉴定出四个 16S rDNA 区域，它们具有高度种间变异，但在各自物种内具有高度保守的序列模式。基于对这些序列模式的分析，开发了一个简单的方案，这使得能够明确识别大多数弯曲杆菌物种。我们推荐 16S rDNA 序列分析作为特异性鉴定弯曲杆菌的有效、快速的方法。
Species-specific identification of campylobacters is problematic, primarily due to the absence of suitable biochemical assays and the existence of atypical strains. 16S rRNA gene (16S rDNA)-based identification of bacteria offers a possible alternative when phenotypic tests fail. Therefore, we evaluated the reliability of 16S rDNA sequencing for the species-specific identification of campylobacters. Sequence analyses were performed by using almost 94% of the complete 16S rRNA genes of 135 phenotypically characterized Campylobacter strains, including all known taxa of this genus. It was shown that 16S rDNA analysis enables specific identification of most Campylobacter species. The exception was a lack of discrimination among the taxa Campylobacter jejuni and C coli and atypical C lari strains, which shared identical or nearly identical 16S rDNA sequences. Subsequently, it was investigated whether partial 16S rDNA sequences are sufficient to determine species identity. Sequence alignments led to the identification of four 16S rDNA regions with high degrees of interspecies variation but with highly conserved sequence patterns within the respective species. A simple protocol based on the analysis of these sequence patterns was developed, which enabled the unambiguous identification of the majority of Campylobacter species. We recommend 16S rDNA sequence analysis as an effective, rapid procedure for the specific identification of campylobacters.