CLONING AND CHARACTERIZATION OF THE GENE ENCODING INORGANIC PYROPHOSPHATASE OF ESCHERICHIA-COLI K-12
CLONING AND CHARACTERIZATION OF THE GENE ENCODING INORGANIC PYROPHOSPHATASE OF ESCHERICHIA-COLI K-12
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DOI：
10.1128/jb.170.12.5901-5907.1988
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发表时间：
1988-12-01
影响因子：
3.2
通讯作者：
HEINONEN, J
中科院分区：
文献类型：
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作者：
LAHTI, R;PITKARANTA, T;HEINONEN, J
克隆了大肠杆菌K-12无机焦磷酸酶（PPase）基因ppa的全序列。通过引物延伸作图鉴定ppa mRNA的5“末端。典型的E. coli sigma 70启动子在mRNA 5“末端的上游被鉴定。ppa的结构基因含有528个碱基对，由其推导出175个氨基酸的翻译产物，Mr 19，752。推导的氨基酸组成与先前确定的PPase的氨基酸组成完全吻合（P. Burton，D. C. Hall和J. Josse，J.Biol.Chem.245：4346-4351，1970）。此外，还对E. coliPPase的活性。A.科恩（Ph.D.论文，芝加哥大学，1978）与从核苷酸序列推断的相同。这是首次报道PPase基因的克隆。
Escherichia coli K-12 gene ppa encoding inorganic pyrophosphatase (PPase) was cloned and sequenced. The 5'' end of the ppa mRNA was identified by primer extension mapping. A typical E. coli sigma 70 promoter was identified immediately upstream of the mRNA 5'' end. The structural gene of ppa contains 528 base pairs, from which a 175-amino-acid translation product, Mr 19,752, was deduced. The deduced amino acid composition perfectly fitted with that of PPase as previously determined (P. Burton, D. C. Hall, and J. Josse, J. Biol. Chem. 245:4346-4351, 1970). Furthermore, the partial amino acid sequence (residues 1 to 108) of E. coli PPase determined by S. A. Cohen (Ph.D. thesis, University of Chicago, 1978) was the same as that deduced from the nucleotide sequence. This is the first report of the cloning of a PPase gene.