Plasmodium falciparum Produce Lower Infection Intensities in Local versus Foreign Anopheles gambiae Populations
Plasmodium falciparum Produce Lower Infection Intensities in Local versus Foreign Anopheles gambiae Populations
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DOI：
10.1371/journal.pone.0030849
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发表时间：
2012-01-26
期刊：
影响因子：
3.7
通讯作者：
Cohuet, Anna
中科院分区：
文献类型：
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作者：
Harris, Caroline;Morlais, Isabelle;Cohuet, Anna
恶性疟原虫和冈比亚按蚊在非洲的基因组成和种群动态都表现出极大的多样性。已知个体蚊子和寄生虫的遗传学在确定载体感染的结果中起作用，但感染表型的差异是否在种群之间变化仍有待研究。在这里，我们建立了两个A。冈比亚公司来自喀麦隆和布基纳法索的M分子形式菌落，代表每个地理位置的本地和外国人口。在喀麦隆和布基纳法索使用当地野生恶性疟原虫对两个菌落进行实验感染，在每个地点产生同域和异域的媒介-寄生虫组合。感染表型确定的卵囊的患病率和强度，至少有9个感染的每一个载体寄生虫组合。发现同域感染比异域感染每中肠产生的卵囊少25%，而流行率不受本地/外来相互作用的影响。在同域分布的夫妇卵囊数量的减少可能是进化过程的结果，蚊子种群在当地适应了他们的寄生虫种群。未来的研究媒介-寄生虫相互作用必须考虑到这里揭示的地理适应规模进行实验，在自然同域人群，流行病学上有意义的结果。
Both Plasmodium falciparum and Anopheles gambiae show great diversity in Africa, in their own genetic makeup and population dynamics. The genetics of the individual mosquito and parasite are known to play a role in determining the outcome of infection in the vector, but whether differences in infection phenotype vary between populations remains to be investigated. Here we established two A. gambiae s.s. M molecular form colonies from Cameroon and Burkina Faso, representing a local and a foreign population for each of the geographical sites. Experimental infections of both colonies were conducted in Cameroon and Burkina Faso using local wild P. falciparum, giving a sympatric and allopatric vector-parasite combination in each site. Infection phenotype was determined in terms of oocyst prevalence and intensity for at least nine infections for each vector-parasite combination. Sympatric infections were found to produce 25% fewer oocysts per midgut than allopatric infections, while prevalence was not affected by local/foreign interactions. The reduction in oocyst numbers in sympatric couples may be the result of evolutionary processes where the mosquito populations have locally adapted to their parasite populations. Future research on vector-parasite interactions must take into account the geographic scale of adaptation revealed here by conducting experiments in natural sympatric populations to give epidemiologically meaningful results.