Linker histone protects linker DNA on only one side of the core particle and in a sequence-dependent manner
Linker histone protects linker DNA on only one side of the core particle and in a sequence-dependent manner
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DOI：
10.1073/pnas.95.7.3396
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发表时间：
1998-03-31
影响因子：
11.1
通讯作者：
Zlatanova, J
中科院分区：
文献类型：
--
作者：
An, WJ;Leuba, SH;Zlatanova, J
在两个重组系统中，定量测量了连接基组蛋白(H1度)对染色体DNA的微球菌核酸酶消化的保护作用。我们使用了在包含Lytechinus varegatus 5S rRNA基因的DNA片段上的两个不同位置重组的染色体小体，以及在包含Ga14和USF结合位点的序列上的特定位置，在所有情况下，我们都发现了不对称保护，大约20通过保护核心颗粒的一侧而不保护另一侧。我们通过交联实验证明，这一结果不是由于连接子组蛋白添加或微球菌核酸酶裂解引起的组蛋白核心的任何滑动，因为核心颗粒本身是它的对称对象，连接子组蛋白的首选不对称位置必须由DNA序列中的未知元件决定。
The protection against micrococcal nuclease digestion afforded to chromatosomal DNA by the presence of a linker histone (H1 degrees) has been quantitatively measured in two reconstituted systems. We have used chromatosomes reconstituted at two distinct positions on a DNA fragment containing the 5S rRNA gene from Lytechinus variegatus and at a specific position on a sequence containing Ga14- and USF-binding sites, In all cases, we find asymmetric protection, with approximate to 20 by protected an one side of the core particle and no protection on the other. We demonstrated through crosslinking experiments that the result is not due to any sliding of the histone core caused by either linker histone addition or micrococcal nuclease cleavage, Because the core particle is itself it symmetric object, the preferred asymmetric location of a linker histone must be dictated by unknown elements in the DNA sequence.