Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits.
Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits.
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DOI：
10.1016/j.biomaterials.2017.05.021
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发表时间：
2017-08
期刊：
影响因子：
14
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Du Y;Liu H;Yang Q;Wang S;Wang J;Ma J;Noh I;Mikos AG;Zhang S
由于软骨存在复杂的层次结构和缺乏血液供应，骨软骨缺损无法充分自我修复。因此，该领域剩下的主要挑战之一是满足骨软骨修复特定要求的仿生支架的结构设计。为了解决这个障碍，通过选择性激光烧结（SLS）技术构建了由聚（ε-己内酯）（PCL）和羟基磷灰石（HA）/PCL微球组成的仿生多层骨软骨支架。 SLS 衍生的支架表现出优异的生物相容性，可支持体外细胞粘附和增殖。通过将脱细胞多层支架植入兔骨软骨缺损模型来评估修复效果。我们的研究结果表明，多层支架能够通过加速早期软骨下骨再生来诱导关节软骨形成，并且新形成的组织能够与天然组织很好地整合。因此，当前的研究不仅实现了骨软骨修复，而且还提出了一种通过 SLS 技术制造具有精心设计的结构和梯度成分的仿生多层支架的有前景的策略。
Osteochondral defects cannot be adequately self-repaired due to the presence of the sophisticated hierarchical structure and the lack of blood supply in cartilage. Thus, one of the major challenges remaining in this field is the structural design of a biomimetic scaffold that satisfies the specific requirements for osteochondral repair. To address this hurdle, a bio-inspired multilayer osteochondral scaffold that consisted of the poly(ε-caprolactone) (PCL) and the hydroxyapatite (HA)/PCL microspheres, was constructed via selective laser sintering (SLS) technique. The SLS-derived scaffolds exhibited an excellent biocompatibility to support cell adhesion and proliferation in vitro. The repair effect was evaluated by implanting the acellular multilayer scaffolds into osteochondral defects of a rabbit model. Our findings demonstrated that the multilayer scaffolds were able to induce articular cartilage formation by accelerating the early subchondral bone regeneration, and the newly formed tissues could well integrate with the native tissues. Consequently, the current study not only achieves osteochondral repair, but also suggests a promising strategy for the fabrication of bio-inspired multilayer scaffolds with well-designed architecture and gradient composition via SLS technique.
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