Direct vascular actions of quercetin in aorta from renal hypertensive rats.
Direct vascular actions of quercetin in aorta from renal hypertensive rats.
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DOI：
10.1016/j.krcp.2015.12.003
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发表时间：
2016-03
影响因子：
3
通讯作者：
Yeum CH
中科院分区：
文献类型：
--
作者：
Choi S;Ryu KH;Park SH;Jun JY;Shin BC;Chung JH;Yeum CH
在高血压动物模型中，已知长期服用膳食类黄酮槲皮素可降低血压并恢复内皮功能障碍。本研究探讨了槲皮素对慢性2肾1夹（2K 1C）肾性高血压大鼠血管反应的直接影响。抗氧化剂维生素抗坏血酸对血管反应性的影响也进行了检查。通过夹闭左肾动脉诱导2K 1C肾性高血压;年龄匹配的接受假治疗的大鼠作为对照。将胸椎固定在组织浴中，用于测量等长张力。与假手术大鼠相比，2K 1C大鼠对乙酰胆碱的舒张反应明显减弱。槲皮素或抗坏血酸增强了2K 1C大鼠乙酰胆碱诱导的舒张作用，而假手术大鼠中没有观察到显着差异。2K 1C和假手术大鼠对硝普钠的舒张反应相当，硝普钠诱导的舒张在两组中均未被槲皮素或抗坏血酸改变。与假手术组相比，2K 1C组大鼠对苯肾上腺素的收缩反应明显增强。在2K 1C大鼠中，用槲皮素或抗坏血酸预处理抑制苯肾上腺素诱导的收缩，而在假手术大鼠中，两种化学物质都不影响反应。N-硝基-L-精氨酸甲酯显著增强假手术大鼠对苯肾上腺素的收缩反应，而在2K 1C大鼠中没有观察到显著差异。在2K 1C或假手术大鼠中，在存在N-硝基-L-精氨酸甲酯的情况下，槲皮素或抗坏血酸不影响苯肾上腺素诱导的收缩。急性暴露于槲皮素似乎改善内皮依赖性舒张和抑制收缩反应，类似于抗坏血酸在2K 1C高血压中的作用。这些结果部分解释了槲皮素在肾性高血压中的血管有益作用。
Chronic treatment with the dietary flavonoid quercetin is known to lower blood pressure and restore endothelial dysfunction in animal models of hypertension. This study investigated the direct effects of quercetin on vascular response in chronic 2-kidney, 1-clip (2K1C) renal hypertensive rats. The effects of antioxidant vitamin ascorbic acid on the vasoreactivity were also examined. 2K1C renal hypertension was induced by clipping the left renal artery; age-matched rats that received sham treatment served as controls. Thoracic aortae were mounted in tissue baths for the measurement of isometric tension. Relaxant responses to acetylcholine were significantly attenuated in 2K1C rats in comparison with sham rats. Quercetin or ascorbic acid augmented acetylcholine-induced relaxation in 2K1C rats, whereas no significant differences were noted in sham rats. The relaxation response to sodium nitroprusside was comparable between 2K1C and sham rats, and sodium nitroprusside–induced relaxation was not altered by quercetin or ascorbic acid in either group. The contractile response to phenylephrine was significantly enhanced in 2K1C rats compared with sham rats. Phenylephrine-induced contraction was inhibited by pretreatment with quercetin or ascorbic acid in 2K1C rats, whereas neither chemical affected responses in sham rats. Nw-nitro-L-arginine methyl ester markedly augmented the contractile response to phenylephrine in sham rats, whereas no significant differences were observed in 2K1C rats. Quercetin or ascorbic acid did not affect phenylephrine-induced contraction in the presence of Nw-nitro-L-arginine methyl ester in either 2K1C or sham rats. Acute exposure to quercetin appears to improve endothelium-dependent relaxation and inhibit the contractile response, similar to the effect of ascorbic acid in 2K1C hypertension. These results partially explain the vascular beneficial effects of quercetin in renal hypertension.