Protective effects of bilberry (Vaccinium myrtillus L.) extract on restraint stress-induced liver damage in mice
Protective effects of bilberry (Vaccinium myrtillus L.) extract on restraint stress-induced liver damage in mice
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DOI：
10.1021/jf800728m
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发表时间：
2008-09-10
影响因子：
6.1
通讯作者：
Kurihara, Hiroshi
中科院分区：
文献类型：
--
作者：
Bao, Li;Yao, Xin-Sheng;Kurihara, Hiroshi
实验结果表明，18 h束缚应激可引起严重的肝损伤，血浆丙氨酸氨基转移酶（ALT）水平升高（107-68 +/- 3.19 U/L vs 18.08 +/- 1.46 U/L）。同时，我们观察到与饥饿小鼠相比，束缚小鼠血浆和肝脏中丙二醛（MDA）水平升高，氧自由基吸收能力（ORAC）值降低。以50、100和200 mg/（kg. d）的剂量对小鼠口服施用越橘提取物（含有42.04%花青素）5天，其在200 mg/（kg. d）的剂量下显著降低血浆ALT水平至17.23 +/- 2.49 U/L，从而减轻应激诱导的肝损伤。此外，越橘提取物增加谷胱甘肽（GSH）和维生素C水平，并显着降低MDA和一氧化氮（NO）水平的肝组织。以上结果提示，越橘提取物通过清除自由基和抑制脂质过氧化作用对束缚应激性肝损伤具有保护作用。这项研究表明，越橘提取物通过其抗氧化作用对健康有益。
Our experiments showed that 18 h restraint stress could induce serious liver damage, with an increase in plasma alanine aminotransferase (ALT) level (107-68 +/- 3.19 U/L vs 18.08 +/- 1.46 U/L. Meanwhile, we observed increased malondialdehyde (MDA) levels and lowered oxygen radical absorbance capacity (ORAC) values in plasma and liver of restraint mice compared with starved mice. Bilberry extract (containing 42.04% anthocyanins) was oral administrated to mice at 50, 100, and 200 mg/(kg.day) for five days, which remarkably decreased plasma ALT level to 17.23 +/- 2.49 U/L at the dose of 200 mg/(kg.day) and thus alleviated stress-induced liver damage. In addition, bilberry extracts increased glutathione (GSH) and vitamin C levels and significantly decreased MDA and nitric oxide (NO) levels in the liver tissues. These results suggest that bilberry extract plays an important role in protecting against restraint stress-induced liver damage by both scavenging free radicals activity and lipid peroxidation inhibitory effect. This study showed the beneficial health effects of bilberry extract through its antioxidative action.