Tonsil surface epithelium is ideal for monitoring Ki-67 immunohistochemical staining
Tonsil surface epithelium is ideal for monitoring Ki-67 immunohistochemical staining
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DOI：
10.1111/his.12228
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发表时间：
2013-12-01
期刊：
影响因子：
6.4
通讯作者：
Ho, Donald Ming-Tak
中科院分区：
文献类型：
--
作者：
Hsu, Chih-Yi;Yang, Ching-Fen;Ho, Donald Ming-Tak
方法与结果研究了扁桃体(60例)、宫颈(31例)、乳房皮肤(26例)、食道(15例)、胃(15例)、小肠(15例)和结肠(16例)等多种组织，寻找理想的对照组织。扁桃体表面上皮优于其他组织，因为它显示出易于识别的Ki-67染色模式，包括高阳性(基底层)、低阳性(中间层)和阴性(基底层和浅层)区。大多数中间细胞呈中等至弱染色可作为阳性染色的阈值。在可能影响染色结果的变量中，抗原修复的预处理液的影响最大，其中pH为9的EDTA远好于pH为6的柠檬酸盐溶液。结论扁桃体表面上皮细胞是监测Ki-67染色的有效对照。获得最佳的染色结果可以最大限度地减少由于不同实验室使用的染色方法的差异而导致的Ki-67指数的差异。
AimsTo identify an easily obtainable non-neoplastic tissue that can be used as control material for monitoring optimal Ki-67 immunohistochemical staining.Methods and resultsVarious tissues, including tonsil (60), uterine cervix (31), breast skin (26), oesophagus (15), stomach (15), small intestine (15) and colon (16), were studied in the search for ideal control tissue. Tonsil surface epithelium is superior to other tissues because it displayed an easily recognized Ki-67 staining pattern including high positive (parabasal layer), low positive (intermediate layer) and negative (basal and superficial layers) zones. Moderate to weak staining of the majority of the intermediate cells could serve as a threshold for positive staining. Of the variables potentially affecting staining results that were tested, the pretreatment solution for antigen retrieval had the greatest impact, of which pH 9 EDTA was far better than pH 6 citrate solution.ConclusionsTonsil surface epithelium is a useful control for monitoring Ki-67 staining. Achieving optimal staining results could minimize variations in Ki-67 index due to differences in the staining methods used by different laboratories.