Analysis of the metabolome and transcriptome of Brassica carinata seedlings after lithium chloride exposure
Analysis of the metabolome and transcriptome of Brassica carinata seedlings after lithium chloride exposure
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DOI：
10.1016/j.plantsci.2009.03.013
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发表时间：
2009-07-01
期刊：
影响因子：
5.2
通讯作者：
Gruber, Margaret Y.
中科院分区：
文献类型：
--
作者：
Li, Xiang;Gao, Peng;Gruber, Margaret Y.
B的暴露。在褐籽和黄籽近等基因系中，随着非生理离子锂浓度的增加，对种子发芽率、根长、叶绿素含量和鲜重的影响显著。金属含量分析和植物化学分析表明，锂超积累和脂质和酚类成分显着变化，在棕色种子幼苗。在这里，芥子酸酯和叶绿体脂质取代苯甲酸酯衍生物，白藜芦醇和氧脂锂暴露后。相比之下，黄色种子的植物保持相同的酚类和脂质组合物之前和之后暴露于锂，并不耐受高金属浓度所耐受的棕色种子线。使用甘蓝型油菜15，000表达序列标签（EST）阵列进行的微阵列分析表明，在B处理后，棕色种子系中共有89个基因和黄色种子系中共有95个基因的差异表达超过20倍。氯化锂处理的龙须菜幼苗中有超过1083个基因的表达变化大于2倍。这些差异表达的基因包括与防御、初级代谢、转录、运输、次级代谢、细胞色素P450有关的蛋白以及功能未知的蛋白。转录组在黄色种子和棕色种子B之间变化。氯化锂处理后的龙骨幼苗表明两个品系对锂处理的反应不同。表达模式一般支持植物化学数据。从这项研究的结果来看，B。carinatabrown-seeded种质显示出在中等高浓度的氯化锂（>150 mM）下存活的能力。积累这种金属离子的能力表明棕色种子B。龙骨在锂污染水体和土壤的植物修复中具有一定的潜力。皇冠版权所有（C）2009由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Exposure of B. carinata seedlings to increasing concentrations of a non-physiological ion, lithium, showed significant effects on the germination rate, root length, chlorophyll content and fresh weight in brown-seeded and yellow-seeded near-isogenic lines. Metal content analysis and phytochemical profiling indicated that lithium hyper-accumulated and the lipid and phenolic composition dramatically changed in brown-seeded seedlings. Here, sinapic acid esters and chloroplast lipid were replaced by benzoate derivatives, resveratrol and oxylipins after lithium exposure. In contrast, the yellow-seeded plants maintained the same phenolic and lipid composition before and after exposure to lithium and did not tolerate the high metal concentrations tolerated by the brown-seeded line. Microarray analysis using a Brassica napus 15,000 expressed sequence tag (EST) array indicated a total of 89 genes in the brown-seeded line and 95 genes in the yellow-seeded line were differentially expressed more than 20-fold after treatment of B. carinata seedlings with lithium chloride and more than 1083 genes with expression changes greater than 2-fold. The putative functions of the differentially expressed genes included proteins involved in defense, primary metabolism, transcription, transportation, secondary metabolism, cytochrome P450, as well as proteins with unknown functions. Transcriptome changes between yellow-seeded and brown-seeded B. carinata seedlings after lithium chloride exposure indicated that the two lines responded differently to lithium treatment. The expression patterns generally supported the phytochemical data. From the results of this study, B. carinata brown-seeded germplasm showed an ability to survive under moderately high concentrations of lithium chloride (>150 mM). The ability to accumulate this metal ion suggests brown-seeded B. carinata has some potential in phytoremediation of lithium-contaminated water and soil. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. All rights reserved.