Imaging the electric field associated with mouse and human skin wounds
Imaging the electric field associated with mouse and human skin wounds
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DOI：
10.1111/j.1524-475x.2008.00389.x
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发表时间：
2008-05-01
影响因子：
2.9
通讯作者：
Smith, Peter J. S.
中科院分区：
文献类型：
--
作者：
Nuccitelli, Richard;Nuccitelli, Pamela;Smith, Peter J. S.
我们已经开发了一种非侵入性的仪器，称为生物电场成像仪（BFI），用于映射在小鼠和人类皮肤的伤口附近的表皮和角质层之间的电场。BFI不接触皮肤，而是在皮肤上方的空气中以180 μ m的位移振动一个小金属探针，以通过电容耦合检测表皮的表面电位。在这里，我们描述了我们的第一个应用程序的BFI测量角质层和表皮之间的电场在小鼠皮肤伤口的边缘。我们在伤口处立即测量到177 +/- 14（61）mV/mm的电场，并且场线在伤口周围的所有方向上都指向远离伤口的方向。因为伤口电流在伤口处立即流动，这是指示皮肤损伤的第一个信号。该电场通过损伤电流的向外流动在表皮的外表面处产生。在多层表皮内必须有大小相等且方向相反的电流流动，以在伤口部位产生具有负极的表皮内场。因为在多层表皮内流动的电流分布在更大的区域上，所以预期在该区域中产生的电流密度和随后的E场小于通过角质层下方的BFI测量的电流密度和随后的E场。角质层下的电场通常保持在150-200 mV/mm的范围内3天，然后在接下来的几天内开始下降，一旦伤口愈合完成就下降到零。在伤口附近皮肤应用钠通道阻滞剂阿米洛利后，平均伤口场强降低64 +/- 7%，在应用Cl-通道激活剂前列腺素E2后，平均伤口场强增加82 +/- 21%。
We have developed a noninvasive instrument called the bioelectric field imager (BFI) for mapping the electric field between the epidermis and the stratum corneum near wounds in both mouse and human skin. Rather than touching the skin, the BFI vibrates a small metal probe with a displacement of 180 mu m in air above the skin to detect the surface potential of the epidermis through capacitative coupling. Here we describe our first application of the BFI measuring the electric field between the stratum corneum and epidermis at the margin of skin wounds in mice. We measured an electric field of 177 +/- 14 (61) mV/mm immediately upon wounding and the field lines pointed away from the wound in all directions around it. Because the wound current flows immediately upon wounding, this is the first signal indicating skin damage. This electric field is generated at the outer surface of the epidermis by the outward flow of the current of injury. An equal and opposite current must flow within the multilayered epidermis to generate an intraepidermal field with the negative pole at the wound site. Because the current flowing within the multilayered epidermis is spread over a larger area, the current density and subsequent E field generated in that region is expected to be smaller than that measured by the BFI beneath the stratum corneum. The field beneath the stratum corneum typically remained in the 150-200 mV/mm range for 3 days and then began to decline over the next few days, falling to zero once wound healing was complete. The mean wound field strength decreased by 64 +/- 7% following the application of the sodium channel blocker, amiloride, to the skin near the wound and increased by 82 +/- 21% following the application of the Cl- channel activator, prostaglandin E2.