Oral rehydration solution increases SGLT1 and improves dehydration in a mouse heatstroke model.
Oral rehydration solution increases SGLT1 and improves dehydration in a mouse heatstroke model.
复制标题
口服补液溶液可增加 SGLT1 并改善小鼠中暑模型的脱水情况。
DOI：
10.1097/01.ccm.0000528551.63676.f8
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发表时间：
2018
影响因子：
8.8
通讯作者：
Hayashi M.
中科院分区：
文献类型：
--
作者：
Miyamoto K;Ohtaki H;Takayasu H;Maeda A;Sasaki J;Honda K;Dohi K;Hayashi M.
方法：在湿室（环境温度41±0.5℃，相对湿度70-99%）中产生实验性中暑。将雄性C57BL/6J小鼠分为补充30 mg/kg水分组（water, n= 9）和补充30 mg/kg ORS组（ORS, n= 9），脱水3 h。然后进行HE作用60 min。该补充剂通过胃管口服两次，第一次是在脱水后3小时（HE前），第二次是在HE后立即。he后6小时处死小鼠。采集血液和组织标本（肝、小肠、肾）。同时进行血液学（全血细胞计数和血清化学生化检查）和组织病理学（HE染色）检查。采用Real time PCR技术比较小肠共转运体基因（SGLT1、GLUT2）的表达情况。结果：血液学检查中，ORS组BUN、Cre、AST、LDH水平显著降低（p< 0.05）。组织病理学检查显示，ORS组肝损害较轻。肾脏和小肠无差异。ORS组小肠共转运蛋白基因（SGLT1、GLUT2）表达量显著升高（p< 0.05）。结论：补充ORS可增加小肠共转运蛋白基因表达，改善he后小肠脱水。
Methods: Experimental heatstroke was produced in a moisture chamber (Ambient temperature at 41±0.5 C and relative humidity at 70-99%). Male C57BL/6J mice were divided into two groups: a 30 mg/kg water supplement group (Water, n= 9), and a 30 mg/kg ORS supplement group (ORS, n= 9), and were dehydrated for 3 hours. Then, the animals were subjected to HE for 60 min. The supplement was administered orally twice using gastric tube, the first time 3 hours after dehydration (before HE) and then immediately after HE. Mice were sacrificed 6 hours post-HE. Blood and tissue samples (Liver, Small intestine, Kidney) were collected. The hematological (complete blood count and biochemical exam of blood serum chemistry) and histopathological examination using Hematoxyline-Eosin (HE) staining were also performed. Moreover, cotransporter gene expression (SGLT1, GLUT2) of small intestine were compared using Real time PCR.Results: In hematological examination, BUN, Cre, AST and LDH level were significantly lower in the ORS group (p< 0.05). Histopathological examination showed hepatic damage was relatively mild in ORS group. There was no differences in kidney and small intestine. Cotransporter gene expression (SGLT1, GLUT2) of small intestine was significantly increased in the ORS group (p< 0.05).Conclusions: These results suggest that ORS supplementation increased cotransporter gene expression of small intestine and improves dehydration dehydration post-HE.