Pantothenate auxotrophy of Methylobacterium spp. isolated from living plants
Pantothenate auxotrophy of Methylobacterium spp. isolated from living plants
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甲基杆菌属泛酸营养缺陷型。
DOI：
10.1080/09168451.2018.1549935
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yurimoto Hiroya
中科院分区：
文献类型：
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作者：
Yoshida Yusuke;Iguchi Hiroyuki;Sakai Yasuyoshi;Yurimoto Hiroya
一些粉红色的兼性甲基营养菌（PPFM）属于甲基杆菌属。发现从活的植物样品中分离的PPFM需要B族维生素以使其在基本培养基中生长，并且大多数B族维生素营养缺陷型PPFM需要泛酸盐（维生素B5）。利用代表菌株甲基杆菌（Methylobacterium sp.）OR 01证明该菌株不能合成泛酸的前体之一β-丙氨酸。β-丙氨酸和几种泛酸的前体恢复了甲基杆菌的生长。在基本培养基中的OR 01。此外，该菌株还能在不含泛酸盐或其前体的培养基中定殖于拟南芥叶片。在A. Thalianaleaves.这些结果表明，泛酸营养缺陷型PPFM可以共生定殖植物叶片表面，通过获取β-丙氨酸和其他前体，除了泛酸。最后，讨论了PPFM在叶际环境中B族维生素营养缺陷型的适合度优势。
A number of pink-pigmented facultative methylotrophs (PPFMs) belonging toMethylobacteriumspp. isolated from living plant samples were found to require B vitamins for their growth in minimal medium, and most B vitamin-auxotrophic PPFMs required pantothenate (vitamin B5). Further investigation of pantothenate auxotrophy using the representative strainMethylobacteriumsp. OR01 demonstrated that this strain cannot synthesize β-alanine, one of the precursors of pantothenate. β-alanine and several precursors of pantothenate restored the growth ofMethylobacteriumsp. OR01 in minimal medium. Furthermore, this strain could colonize leaves ofArabidopsis thalianacultivated in medium without pantothenate or its precursors. Pantothenate, β-alanine and several precursors were detected in the suspension ofA. thalianaleaves. These results suggest that pantothenate-auxotrophic PPFMs can symbiotically colonize the surface of plant leaves by acquiring β-alanine and other precursors, in addition to pantothenate. Finally, the fitness advantage of B vitamin auxotrophy of PPFMs in the phyllosphere environment is discussed.