A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis.
A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis.
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DOI：
10.1007/bf02670470
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发表时间：
1993-01-01
影响因子：
2.1
通讯作者：
Wendel, J. F.
中科院分区：
文献类型：
--
作者：
Paterson, A. H.;Brubaker, C. L.;Wendel, J. F.
由于棉属植物含有大量的多糖和可氧化醌类物质，从棉属植物中提取高质量的基因组DNA是困难的。描述了一种允许分离适合RFLP和聚合酶链反应分析以及大多数常规克隆应用的大小和质量的棉花基因组DNA的程序。几种抗氧化剂，酚结合剂，酚氧化酶抑制剂在整个过程中使用，大多数多糖在程序的早期通过分离细胞核被消除。
Extraction of high-quality genomic DNA from Gossypium species is difficult due to high levels of polysaccharides and oxidizable quinones. A procedure is described that permits isolation of cotton genomic DNA of a suitable size and quality for RFLP and polymerase chain reaction analysis, as well as most routine cloning applications. Several antioxidants, phenol-binding reagents, and phenol oxidase inhibitors are used throughout the procedure, and most polysaccharides are eliminated early in the procedure by isolation of nuclei.