Minocycline Effects on Intracerebral Hemorrhage-Induced Iron Overload in Aged Rats: Brain Iron Quantification With Magnetic Resonance Imaging.
Minocycline Effects on Intracerebral Hemorrhage-Induced Iron Overload in Aged Rats: Brain Iron Quantification With Magnetic Resonance Imaging.
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DOI：
10.1161/strokeaha.117.019860
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发表时间：
2018-04
期刊：
影响因子：
8.3
通讯作者：
Xi G
中科院分区：
文献类型：
--
作者：
Cao S;Hua Y;Keep RF;Chaudhary N;Xi G
脑铁超载是脑出血后脑损伤的重要因素。本研究采用磁共振成像（MRI）R2* 标测定量脑出血后的脑铁水平。观察米诺环素对老年大鼠铁超载和ICH脑损伤的影响。对老年（18月龄）雄性Fischer 344大鼠脑内注射自体血或生理盐水，并通过MRI R2* 标测测量脑铁水平。一些ICH大鼠用米诺环素或溶剂处理。在ICH后第7天和第28天对大鼠实施安乐死，并将脑用于免疫组织化学和Western印迹分析。在不同的时间点进行MRI（T2加权，T2* 梯度回波和R2* 映射）序列。ICH诱导的血肿周围区域的脑铁过载可以通过R2* 映射来量化。米诺环素治疗减少了脑铁积累、T2* 病变体积、铁处理蛋白上调、神经元细胞死亡和神经功能缺损（p < 0.05）。MRI R2* 标测是一种可靠的、无创的方法，可定量测定脑出血后脑组织铁含量。米诺环素减少老年大鼠ICH相关血肿周围铁积聚和脑损伤
Brain iron overload is a key factor causing brain injury after intracerebral hemorrhage (ICH). This study quantified brain iron levels after ICH with magnetic resonance imaging (MRI) R2* mapping. The effect of minocycline on iron overload and ICH-induced brain injury in aged rats was also determined. Aged (18-month-old) male Fischer 344 rats had an intracerebral injection of autologous blood or saline, and brain iron levels were measured by MRI R2* mapping. Some ICH rats were treated with minocycline or vehicle. The rats were euthanized at day 7 and 28 after ICH and brains used for immunohistochemistry and Western blot analyses. MRI (T2-weighted, T2* gradient-echo and R2* mapping) sequences were performed at different time points. ICH-induced brain iron overload in the perihematomal area could be quantified by R2* mapping. Minocycline treatment reduced brain iron accumulation, T2* lesion volume, iron handling protein upregulation, neuronal cell death, and neurologic deficits (p < 0.05). MRI R2* mapping is a reliable and noninvasive method, which can quantitatively measure brain iron levels after ICH. Minocycline reduced ICH-related perihematomal iron accumulation and brain injury in aged rats.