Metagenomic approaches for direct and cell culture evaluation of the virological quality of wastewater
Metagenomic approaches for direct and cell culture evaluation of the virological quality of wastewater
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DOI：
10.1016/j.jviromet.2014.09.017
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发表时间：
2014-12-15
影响因子：
3.1
通讯作者：
Rose, Joan B.
中科院分区：
文献类型：
--
作者：
Aw, Tiong Gim;Howe, Adina;Rose, Joan B.
基于基因组的分子技术正在成为一种强大的工具，可以全面表征水和废水微生物组。最近，产生大量序列数据的下一代测序（NGS）技术开始影响环境病毒学领域。在这项研究中，NGS和生物信息学已被用于直接检测和表征废水中的病毒和细胞培养后分离的病毒。通过聚乙二醇沉淀法从污水样品中浓缩和纯化病毒颗粒。在使用Illumina技术测序之前提取病毒核酸并随机扩增，产生总共1800万个序列读数。大多数检测到的病毒序列无法表征，表明病毒的多样性尚待发现。这种污水病毒组主要由噬菌体控制，并含有与已知的人类致病性病毒相关的序列，例如腺病毒（种B、C和F）、多瘤病毒JC和BK以及肠道病毒（B型）。还发现了一系列其他动物病毒，表明存在未知的人畜共患病毒。这项研究证明了宏基因组方法在复杂环境水样中表征病毒的可行性。(C)2014爱思唯尔有限公司版权所有。
Genomic-based molecular techniques are emerging as powerful tools that allow a comprehensive characterization of water and wastewater microbiomes. Most recently, next generation sequencing (NGS) technologies which produce large amounts of sequence data are beginning to impact the field of environmental virology. In this study, NGS and bioinformatics have been employed for the direct detection and characterization of viruses in wastewater and of viruses isolated after cell culture. Viral particles were concentrated and purified from sewage samples by polyethylene glycol precipitation. Viral nucleic acid was extracted and randomly amplified prior to sequencing using Illumina technology, yielding a total of 18 million sequence reads. Most of the viral sequences detected could not be characterized, indicating the great viral diversity that is yet to be discovered. This sewage virome was dominated by bacteriophages and contained sequences related to known human pathogenic viruses such as adenoviruses (species B, C and F), polyomaviruses JC and BK and enteroviruses (type B). An array of other animal viruses was also found, suggesting unknown zoonotic viruses. This study demonstrated the feasibility of metagenomic approaches to characterize viruses in complex environmental water samples. (C) 2014 Elsevier B.V. All rights reserved.