Binding of Vibrio anguillarum to neutral glycosphingolipids from intestinal mucosa of rainbow trout (Oncorhynchus mykiss).
Binding of Vibrio anguillarum to neutral glycosphingolipids from intestinal mucosa of rainbow trout (Oncorhynchus mykiss).
复制标题
鳗弧菌与虹鳟鱼（Oncorhynchus mykiss）肠粘膜中性糖鞘脂的结合。
DOI：
10.1292/jvms.66.205
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
H. Kodama
中科院分区：
文献类型：
--
作者：
T. Irie;S. Watarai;T. Iwasaki;H. Kodama
为了检测虹鳟鱼肠粘膜上的鞘糖脂（glycosphingolipids，GSL）是否作为鳗弧菌的结合受体，我们分析了虹鳟鱼肠粘膜上的中性GSL，并研究了细菌与中性GSL的结合。在高效薄层色谱（TLC）板上鉴定了两种中性GSL，命名为N-1和N-2。在TLC免疫染色试验中，鳗弧菌仅与半乳糖神经酰胺（GalCer）、乳糖神经酰胺和N-1结合，其TLC迁移率与GalCer相同，但既不与葡萄糖神经酰胺结合，也不与N-2结合。这些结果表明N-1是GalCer（Gal beta 1-1Cer），并且虹鳟肠粘膜上的N-1（GalCer）充当鳗弧菌的受体。
To test whether glycosphingolipids (GSLs) on the intestinal mucosa of rainbow trout (Oncorhynchus mykiss) serve as a binding receptor for Vibrio anguillarum, we analyzed neutral GSLs from rainbow trout intestinal mucosa and investigated the binding of bacteria to neutral GSLs. Two kinds of neutral GSLs, designated N-1 and N-2, were identified on high-performance thin-layer chromatography (TLC) plates. In TLC immunostaining tests, V. anguillarum bound only to galactosylceramide (GalCer), lactosylceramide and N-1 having the same TLC mobility as GalCer, but neither to glucosylceramide nor to N-2. These results suggest that N-1 is GalCer (Gal beta 1-1Cer) and also that N-1 (GalCer) on rainbow trout intestinal mucosa act as a receptor for V. anguillarum.