EXPRESSION OF BETA-1 INTEGRINS IN NONNEOPLASTIC MAMMARY EPITHELIUM, FIBROADENOMA AND CARCINOMA OF THE BREAST
EXPRESSION OF BETA-1 INTEGRINS IN NONNEOPLASTIC MAMMARY EPITHELIUM, FIBROADENOMA AND CARCINOMA OF THE BREAST
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DOI：
10.1007/bf01621803
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发表时间：
1993-03-01
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影响因子：
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通讯作者：
MOLLER, P
中科院分区：
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作者：
MECHTERSHEIMER, G;MUNK, M;MOLLER, P
应用抗β1和α1~α6亚基的单抗，对正常乳腺、乳腺病变、12例乳腺良性肿瘤和90例乳腺癌组织中的β1整合素进行免疫组织化学检测。与非肿瘤性乳腺和良性肿瘤的上皮细胞相比，癌细胞在α2、α3和α6亚基的表达模式上有明显的定量变化。相反，β1、α1、α4和α5抗原的分布模式与在非肿瘤性乳腺上皮中观察到的情况基本一致。在71.0%的癌组织中发现α2的异常表达，从27.5%的肿瘤细胞数量明显减少到43.5%的肿瘤细胞完全缺失。在癌组织中，39.9%的肿瘤组织中α3的表达有定量变化，15.4%的肿瘤细胞中α3阳性细胞含量异常低，24.5%的病例中该分子完全缺失。73.2%的癌组织中α6蛋白表达异常，31.9%的肿瘤组织中α6蛋白表达阴性，41.3%的肿瘤组织中α6蛋白表达完全缺失。α2和α3亚基的异常低表达/缺失与雌激素受体阴性相关(分别为P<；0.033和P<；0.04)。此外，α2的异常低表达/缺失与肿瘤的低分化有关(P<；0.014)。乳腺癌中β1相关α亚基表达模式的定量变化可能导致细胞-细胞和/或细胞-基质相互作用的紊乱，从而增加肿瘤细胞的侵袭性和迁移性。
Beta1 Integrins were examined immunohistochemically in normal and mastopathic mammary glands, 12 benign tumours and 90 carcinomas of the breast using monoclonal antibodies against beta1 and alpha1 to alpha6 subunits. When compared with epithelial cells of non-neoplastic mammary glands and of benign tumours, carcinoma cells showed considerable quantitative changes in the pattern of alpha2, alpha3 and alpha6 subunit expression. In contrast, the distribution pattern of beta1, alpha1, alpha4 and alpha5 antigens corresponded to the situation observed in non-neoplastic mammary gland epithelium in most instances. An abnormal expression of alpha2 was found in 71.0% of the carcinomas ranging from a remarkably low number of alpha2-positive tumour cells in 27.5% of the cases to a complete absence of the alpha2 molecule in 43.5% of the carcinomas. Of the carcinomas 39.9% exhibited quantitative changes in alpha3 expression with an abnormally low content of alpha3-positive neoplastic cells in 15.4% and a complete absence of this molecule in 24.5% of the cases. Expression of alpha6 was abnormal in 73.2% of the carcinomas, consisting in a greater number of alpha6-negative tumour cells in 31.9% and in a complete absence of alpha6 in 41.3% of the tumours. The abnormally low expression/absence of alpha2 and alpha3 subunits correlated with oestrogen receptor negativity (P < 0.033 and P < 0.04, respectively). In addition, abnormally low expression/absence of alpha2 correlated with poor differentiation of the tumours (P < 0.014). The quantitative changes in the expression pattern of beta1-associated alpha subunits in breast carcinomas may cause a disturbed cell-cell and/or cell-matrix interaction that increases the invasive and migratory property of the tumour cells.