The characteristics of tetraethylammonium-induced rhythmic contractions in canine tracheal smooth muscle: effect of Na-pump inhibition.
The characteristics of tetraethylammonium-induced rhythmic contractions in canine tracheal smooth muscle: effect of Na-pump inhibition.
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四乙铵诱导的犬气管平滑肌节律性收缩的特征：钠泵抑制作用。
DOI：
10.1139/y79-170
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发表时间：
1979
影响因子：
2.1
通讯作者：
Y. Kasuya
中科院分区：
文献类型：
--
作者：
M. Watanabe;Y. Imaizumi;Y. Kasuya
四乙铵离子（TEA）可诱导犬气管平滑肌产生节律性收缩（在TEA浓度为15 mM时，频率为0.09 ± 0.01次收缩/分钟；张力产生为279 ± 36毫克/毫克组织湿重）。代谢耗竭（缺氧、2,4 - 二硝基苯酚（DNP）、碘乙酸（MIA）或氰化钾）可抑制或减弱与各种电活动（例如，振荡或动作电位）耦合的节律性。DNP（3×10⁻⁶ M）或MIA（5×10⁻⁴ M）可完全抑制节律性收缩。缺氧或1 mM氰化钾可使张力产生降低50%。在这些条件下，基础张力不受影响。在无钾的克雷布斯溶液中或在含10⁻⁵ M哇巴因且有10⁻⁷ M阿托品存在的培养基中长时间孵育时，张力产生极小。膜电位在10⁻⁵ M哇巴因中孵育1小时也不受影响。相反，在TEA诱导节律性之后，哇巴因（1×10⁻⁶至3×10⁻⁶ M）在使膜去极化方面特别有效……
Tetraethylammonium ions (TEA) induced rhythmic contractions (RC) in canine tracheal smooth muscle (frequency, 0.09 ± 0.01 contractions/min; tension development, 279 ± 36 mg/mg tissue wet weight, at a TEA concentration of 15 mM). The rhythmicity coupling with various electrical activities (e.g., oscillation or action potentials) was suppressed or reduced by metabolic depletion – anoxia, 2,4-dinitrophenol (DNP), monoiodoacetic acid (MIA), or KCN. DNP (3 × 10−6 M) or MIA (5 × 10−4 M) completely inhibited the RC. Anoxia or 1 mM KCN decreased the tension development by 50%. Under these conditions, basal tension was unaffected. During prolonged incubation in K+-free Krebs solution or in the medium containing 10−5 M ouabain in the presence of 10−7 M atropine, the tension development was minimal. Membrane potential was also unaffected by 1-h incubation with 10−5 M ouabain. In contrast, after the rhythmicity was induced by TEA, ouabain (1 × 10−6 to 3 × 10−6 M) was especially effective in depolarizing the membrane....