Mapping and expression analyses during porcine foetal muscle development of 12 genes involved in histone modifications
Mapping and expression analyses during porcine foetal muscle development of 12 genes involved in histone modifications
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猪胎儿肌肉发育过程中涉及组蛋白修饰的 12 个基因的定位和表达分析
DOI：
10.1111/j.1365-2052.2008.01818.x
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发表时间：
2009-04-01
期刊：
影响因子：
2.4
通讯作者：
Liu, B.
中科院分区：
文献类型：
--
作者：
Peng, Y. B.;Yerle, M.;Liu, B.
组蛋白修饰（甲基化和去甲基化）通过其对染色质结构的作用调节基因表达并在细胞增殖和分化中发挥作用。在此背景下，我们研究了骨骼肌增殖和分化过程中作用于组蛋白甲基化和去甲基化的基因的时间表达谱。使用定量实时PCR来定量胎儿骨骼肌中CARM 1、JARID 1A、JMJD 2A、LSD 1、PRMT 2、PRMT 5、SMYD 1、SMYD 2、SMYD 3、SETDB 1、Suv 39 h2和SUZ 12的mRNA水平。结果表明，CARM 1、JARID 1A、JMJD 2A、SMYD 1和SMYD 2在33 d、65 d和90 d胚胎肌肉中差异表达。将这12个基因分别定位于猪2 q21 -24、5 q25、6 q35、6 q12 -21、6p 15、7 q21、3q 21 -27、9 q26、10 p16、4 q15 -16、10 q14 -16和12 p12染色体上。综合已报道的QTL定位结果、基因表达分析和辐射杂交定位结果，这些结果表明CARM 1、JARID 1A、JMJD 2A、SMYD 1和SMYD 2这5个基因可能是控制生长和背膘厚性状的良好候选基因。
Histone modifications (methylation and demethylation) regulate gene expression and play a role in cell proliferation and differentiation by their actions on chromatin structure. In this context, we studied the temporal expression profiles of genes acting on histone methylation and demethylation during skeletal muscle proliferation and differentiation. Quantitative real-time PCR was used to quantify the mRNA levels of CARM1, JARID1A, JMJD2A, LSD1, PRMT2, PRMT5, SMYD1, SMYD2, SMYD3, SETDB1, Suv39h2 and SUZ12 in foetal skeletal muscle. Our results showed that CARM1, JARID1A, JMJD2A, SMYD1 and SMYD2 were differentially expressed in embryonic muscles of 33 days post-conception (dpc), 65 dpc and 90 dpc. These 12 genes were mapped to porcine chromosomes (SSC) 2q21-24, 5q25, 6q35, 6q12-21, 6p15, 7q21, 3q21-27, 9q26, 10p16, 4q15-16, 10q14-16 and 12p12 respectively. Taking into account the reported QTL mapping results, gene expression analysis and radiation hybrid mapping results, these results suggest that five genes (CARM1, JARID1A, JMJD2A, SMYD1 and SMYD2) could be good candidate genes for growth and backfat thickness traits.