Function of pancreatic endocrine cells in experimental acute pancreatitis
Function of pancreatic endocrine cells in experimental acute pancreatitis
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胰腺内分泌细胞在实验性急性胰腺炎中的功能
DOI：
10.1007/bf01657685
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发表时间：
1982
影响因子：
2.6
通讯作者：
I. Miyazaki
中科院分区：
文献类型：
--
作者：
Y. Kinami;T. Mura;M. Sugii;I. Miyazaki
本实验旨在探讨急性胰腺炎时胰腺内分泌功能的变化。将雄性东柳大鼠分为2组，即Block法诱导的急性胰腺炎组和单纯开腹手术对照组。前一组的组织学检查显示间质性水肿性胰腺炎，但尽管外分泌区有炎症变化，但内分泌细胞无明显改变。急性胰腺炎大鼠胰腺组织中胰岛素和胰高血糖素水平明显低于对照组。使用3 H-亮氨酸的放射自显影研究显示，急性胰腺炎大鼠内分泌颗粒的颗粒计数显著减少。从分离的胰岛释放的胰岛素不减少，直到72小时后，急性胰腺炎的发展，而从分离的胰岛释放胰高血糖素减少6小时后，急性胰腺炎的发展。这些发现表明，在急性胰腺炎中，胰腺内分泌细胞的合成和分泌功能降低，并且表明对A细胞的损害比对B细胞的损害发生得更快。
This experimental study was carried out to clarify changes in pancreatic endocrine function in acute pancreatitis. Male Donryu rats were divided into 2 groups, consisting of a group with acute pancreatitis induced by Block's method, and another group with simple laparotomy as control. Histological examination in the former group revealed an interstitial edematous pancreatitis, but there was no marked alteration of endocrine cells in spite of inflammatory changes in the exocrine region. Insulin and glucagon levels in the pancreatic tissue of rats with acute pancreatitis were significantly lower than those of control rats. Autoradiographic studies using3H‐leucine showed a significant decrease of grain counts for endocrine granules in rats with acute pancreatitis. The release of insulin from isolated islets of Langerhans was not reduced until 72 hours after the development of acute pancreatitis, while the release of glucagon from isolated islets was reduced 6 hours after the development of acute pancreatitis. These findings indicate that there is a reduction of synthesis and secretion function in endocrine cells of the pancreas in acute pancreatitis, and suggest that the damage to A cells occurs more rapidly than damage to B cells.