Gold nanocluster-based fluorescence biosensor for targeted imaging in cancer cells and ratiometric determination of intracellular pH
Gold nanocluster-based fluorescence biosensor for targeted imaging in cancer cells and ratiometric determination of intracellular pH
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基于金纳米簇的荧光生物传感器，用于癌细胞靶向成像和细胞内 pH 值的比例测定
DOI：
10.1016/j.bios.2014.10.034
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发表时间：
2015-03-15
影响因子：
12.6
通讯作者：
Tian, Yang
中科院分区：
文献类型：
--
作者：
Ding, Changqin;Tian, Yang
pH值失调是癌症的标志。这是一个挑战，为开发一种生物传感器，有针对性地成像在癌细胞和监测细胞内的pH值。在这里，一个比例荧光生物传感器的pH值测定开发与有针对性地成像到叶酸受体（FR）丰富的癌细胞在同一时间。以牛血清白蛋白（BSA）保护的AuNCs为参比荧光团，异硫氰酸荧光素（FITC）为pH响应信号，同时与叶酸（FA）偶联，对癌细胞进行靶向成像。所开发的比率式生物传感器可以监测pH与较宽的线性范围从6.0 - 7.8与pKa在6.84。在不同pH条件下，该探针对各种金属离子和氨基酸均表现出较高的选择性，其荧光比基本保持恒定（<5%）。当pH值在6.0和8.0之间切换时，它还显示出良好的循环准确性，以及低细胞毒性。AuNC基无机-有机纳米杂化生物传感器具有良好的细胞渗透性、低细胞毒性和长期光稳定性。据此，利用pH传感器对FR + ve Hela细胞进行靶向成像，并以FR-ve肺癌细胞A549为对照，实现了对Hela细胞pH变化的监测。(C)2014爱思唯尔有限公司版权所有。
The dysregulated pH is working as a mark of cancer. It is a challenge for developing a biosensor for targeted imaging in cancer cells and monitoring of intracellular pH. Here, a ratiometric fluorescence biosensor for pH determination was developed with targeted imaging into folate acceptor (FR)-rich cancer cells at the same time. AuNCs protected by bovine serum albumin (BSA) worked as reference fluorophore and fluorescein-isothiocyanate (FITC) acted as the response signal for pH. For targeted imaging of cancer cells, the AuNCs were simultaneously conjugated with folic acid (FA). The developed ratiometric biosensor can monitor pH with a wide linear range from 6.0-7.8 with a pKa at 6.84. Under every different pH condition, the probe showed high selectivity over various metal ions and amino acids with its fluorescence ratio stayed almost constant ( < 5%). It also showed good cyclic accuracy when pH switched between 6.0 and 8.0, as well as low cytotoxicity. The AuNC-based inorganic-organic nanohybrid biosensor showed good cell-permeability, low cytotoxicity, and long-term photostability. Accordingly, the pH biosensor was employed to gain targeted imaging in FR+ve Hela cells with FR-ve lung carcinoma cells A549 as comparison, and achieved to monitor the pH changes in Hela cells. (C) 2014 Elsevier B.V. All rights reserved.