GROWTH IN MOUSE PERITONEAL-MACROPHAGES OF YERSINIA-PESTIS LACKING ESTABLISHED VIRULENCE DETERMINANTS
GROWTH IN MOUSE PERITONEAL-MACROPHAGES OF YERSINIA-PESTIS LACKING ESTABLISHED VIRULENCE DETERMINANTS
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DOI：
10.1128/iai.45.3.649-654.1984
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
HARMON, PA
中科院分区：
文献类型：
--
作者：
STRALEY, SC;HARMON, PA
培养的小鼠腹腔巨噬细胞用Y.鼠疫的不同之处仅在于不存在≥为该物种确定的毒力决定因素之一。鸟嘌呤营养缺陷型（Pur-）耶尔森氏菌无法在巨噬细胞内存活;外源性次黄嘌呤和鸟苷允许细胞内生长。显然，由于不能在宿主组织中获得足够的游离嘌呤以用于生长和维持，因此Puryersiniae是无毒的，并且表明净生物合成对于抵消巨噬细胞的细胞内杀微生物环境是必需的。在含有染料刚果红（Pgm-）或缺乏与pesticin或Ca依赖性毒力决定簇相关的质粒（分别为Pst-和Vwa-）的培养基中不能着色的耶尔森氏菌被有效地吸收到巨噬细胞中，并在这些细胞内生长，就像所有侵袭性毒力决定簇完整的细菌一样。在感染巨噬细胞之前用100%同源正常血清调理不影响Pgm-或Vwa-Pgm-耶尔森氏菌在巨噬细胞内生长的能力。独立于这些毒力决定因子的属性介导耶尔森氏菌血清和驻留巨噬细胞内的存活和生长，并且除血清和巨噬细胞之外的哺乳动物环境的组分必须与Pgm-、Pst-和Vwa-耶尔森氏菌相互作用以导致它们在体内无毒力。
Cultured mouse resident peritoneal macrophages were challenged with strains of Y. pestis differing only with respect to the absence of .gtoreq. 1 of the virulence determinants established for the species. Guanine-auxotrophic (Pur-) yersiniae were unable to survive within the macrophages; exogenous hypoxanthine and guanosine permitted intracellular growth. Evidently, Pur- yersiniae are avirulent due to inability to obtain sufficient free purines in host tissues for growth and maintenance and indicates that net biosynthesis is necessary to counteract the intracellular microbicidal environment of macrophages. Yersiniae unable to pigment in medium containing the dye Congo red (Pgm-) or lacking either of the plasmids associated with the pesticin or Ca dependence virulence determinants (Pst- and Vwa-, respectively) were taken up as efficiently into macrophages and grew as well within these cells as did bacteria having all invasive virulence determinants intact. Opsonization with 100% homologous normal serum before infection of macrophages did not affect the ability of Pgm- or Vwa- Pgm- yersiniae to grow within macrophages. Attributes independent of these virulence determinants mediate the survival and growth of yersiniae serum and within resident macrophages, and components of the mammalian environment other than serum and macrophages must interact with Pgm-, Pst- and Vwa- yersiniae to cause their avirulence in vivo.